Glucose transporters and transport kinetics in retinoic acid-differentiated T47D human breast cancer cells

1  Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100; and 2  Department of Pathology, Sheba Medical Center, Tel-Hashomer 52621, Israel The rates of glucose transport and of glycolysis and the expression of the glucose transporters GLUT-1 through GLUT-4 were measured i...

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Published in:American journal of physiology: endocrinology and metabolism 2000-09, Vol.279 (3), p.E508-E519
Main Authors: Rivenzon-Segal, Dalia, Rushkin, Edna, Polak-Charcon, Sylvie, Degani, Hadassa
Format: Article
Language:English
Subjects:
Algorithms
Breast Neoplasms - pathology
Breast Neoplasms - ultrastructure
Cell Cycle - drug effects
Cell Differentiation - drug effects
Flow Cytometry
Fluorescent Antibody Technique
Glucose - metabolism
Glucose Transporter Type 1
Glucose Transporter Type 2
Glucose Transporter Type 3
Humans
Keratins - biosynthesis
Kinetics
Magnetic Resonance Spectroscopy
Microscopy, Electron
Monosaccharide Transport Proteins - biosynthesis
Monosaccharide Transport Proteins - genetics
Monosaccharide Transport Proteins - metabolism
Nerve Tissue Proteins
Phosphates - metabolism
Tretinoin - pharmacology
Tumor Cells, Cultured
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Summary:1  Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100; and 2  Department of Pathology, Sheba Medical Center, Tel-Hashomer 52621, Israel The rates of glucose transport and of glycolysis and the expression of the glucose transporters GLUT-1 through GLUT-4 were measured in T47D human breast cancer cells that underwent differentiation by retinoic acid. Glucose transport was found to be the rate-limiting step of glycolysis in control and differentiated cells. The transporters GLUT-1, GLUT-3, and GLUT-4 were present in the cell membrane and in the cytoplasm, and GLUT-2 was present solely in the cytoplasm. Differentiation led to a reduction in GLUT-1 and to an increase in cytoplasmic GLUT-2 and GLUT-3 with no change in GLUT-4. Differentiation also caused a reduction in the maximal velocity of glucose transport by ~40% without affecting the Michaelis-Menten constant of glucose transport. These changes did not alter the steady-state concentration of the phosphate metabolites regulating cell energetics but increased the content of phospholipid breakdown phosphodiesters. In conclusion, differentiation of human breast cancer cells appears to be associated with decreased glycolysis by a mechanism that involves a reduction in GLUT-1 and a slowdown of glucose transport. glycolysis; magnetic resonance spectroscopy; 2-deoxy- D -glucose; GLUT-1
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.2000.279.3.e508