Free leptin is increased in normal pregnancy and further increased in preeclampsia

We measured bound and free leptin levels in preeclamptic and matched normal pregnant and never-pregnant women to determine whether the free component of leptin is increased during pregnancy and further increased in preeclampsia. Two milliliters of serum was obtained from 18 normal and 18 preeclampti...

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Bibliographic Details
Published in:Metabolism, clinical and experimental clinical and experimental, 2000-08, Vol.49 (8), p.1043-1048
Main Authors: Teppa, Roberto J., Ness, Roberta B., Crombleholme, William R., Roberts, James M.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Body Mass Index
Diseases of mother, fetus and pregnancy
Female
Gynecology. Andrology. Obstetrics
Humans
Leptin - blood
Medical sciences
Pre-Eclampsia - blood
Pregnancy - blood
Pregnancy. Fetus. Placenta
Protein Binding
Reference Values
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Summary:We measured bound and free leptin levels in preeclamptic and matched normal pregnant and never-pregnant women to determine whether the free component of leptin is increased during pregnancy and further increased in preeclampsia. Two milliliters of serum was obtained from 18 normal and 18 preeclamptic patients matched by pre-pregnancy body mass index (BMI), and from 18 never-pregnant women matched by BMI with the pregnant groups. The sample was subjected to gel filtration using Sephadex G-100. Radioimmunoassay (RIA) was performed on all fractions, and the proportions of bound and free leptin were determined by analyzing the areas under the curve of the chromatographic profile. The total maternal serum leptin concentration was significantly higher in normal pregnancy compared with the nonpregnant state and was further increased in preeclampsia (33.8 ± 4.1 v 15.2 ± 1.8 ng/mL, P = .002, and 48.1 ± 5.6 ng/mL, P = .02, respectively). Free leptin was increased in normal pregnant compared with never-pregnant women (25.9 ± 4.1 v 11.0 ± 2.0 ng/mL, respectively, P = .01), while the increase of total leptin in preeclampsia was exclusively in the free fraction that was significantly higher versus the normal pregnant group (41.8 ± 5.6 v 25.9 ± 4.1 ng/mL, respectively, P = .01). The bound leptin fraction, by contrast, was significantly increased in the normal pregnant group compared with the preeclamptic group and the never-pregnant group (7.9 ± 0.56 v 6.2 ± 0.36 and 4.1 ± 0.36 ng/mL, respectively, P = .009 and P =
ISSN:0026-0495
1532-8600
DOI:10.1053/meta.2000.7707