Purification of an ACE Inhibitory Peptide after Hydrolysis of Sunflower (Helianthus annuus L.) Protein Isolates

Sunflower protein isolates and the proteases pepsin and pancreatin were used for the production of protein hydrolysates that inhibit angiotensin-I converting enzyme (ACE). Hydrolysates obtained after 3 h of incubation with pepsin and 3 h with pancreatin were studied. An ACE inhibitory peptide with t...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2004-04, Vol.52 (7), p.1928-1932
Main Authors: Megías, Cristina, Yust, Maria del Mar, Pedroche, Justo, Lquari, Hassan, Girón-Calle, Julio, Alaiz, Manuel, Millán, Francisco, Vioque, Javier
Format: Article
Language:English
Subjects:
Angiotensin-Converting Enzyme Inhibitors - isolation & purification
Biological and medical sciences
Chromatography, Gel
Chromatography, High Pressure Liquid
General pharmacology
Helianthus - chemistry
Hydrolysis
Medical sciences
Pancreatin - metabolism
Pepsin A - metabolism
Peptides - isolation & purification
Peptides - metabolism
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Proteins - chemistry
Plant Proteins - metabolism
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Summary:Sunflower protein isolates and the proteases pepsin and pancreatin were used for the production of protein hydrolysates that inhibit angiotensin-I converting enzyme (ACE). Hydrolysates obtained after 3 h of incubation with pepsin and 3 h with pancreatin were studied. An ACE inhibitory peptide with the sequence Phe-Val-Asn-Pro-Gln-Ala-Gly-Ser was obtained by G-50 gel filtration chromatography and high-performance liquid chromatography C18 reverse phase chromatography. This peptide corresponds to a fragment of helianthinin, the 11S globulin from sunflower seeds, which is the main storage protein in sunflower. These results show that sunflower seed proteins are a potential source of ACE inhibitory peptides when hydrolyzed with pepsin and pancreatin. Keywords: Sunflower protein hydrolysate; ACE inhibitors; bioactive peptides; Helianthus annuus L.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf034707r