In vitro behavior of osteoblastic cells cultured in the presence of pseudowollastonite ceramic

Pseudowollastonite ceramic (psW) is a bioactive ceramic that binds to bone when implanted in vivo and may be useful for the treatment of skeletal defects. However, there have been no studies that examined the interaction between psW and osteoblastic cells in vitro. This study investigated the suitab...

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Bibliographic Details
Published in:Journal of biomedical materials research 2004-05, Vol.69A (2), p.351-358
Main Authors: Sarmento, Carlos, Luklinska, Zofia B., Brown, Lindsey, Anseau, Michel, De Aza, Piedad N., De Aza, Salvador, Hughes, Francis J., McKay, Ian J.
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials
biomaterials
bone regeneration
Calcium Compounds
Cell Adhesion - physiology
Cell Division - physiology
Microscopy, Electron, Scanning
osteoblasts
Osteoblasts - cytology
Osteoblasts - physiology
osteoinduction
pseudowollastonite
Rats
Silicates
wollastonite
X-Ray Diffraction
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Summary:Pseudowollastonite ceramic (psW) is a bioactive ceramic that binds to bone when implanted in vivo and may be useful for the treatment of skeletal defects. However, there have been no studies that examined the interaction between psW and osteoblastic cells in vitro. This study investigated the suitability of psW as a substratum for cell attachment and the ability of the material to effect osteoblasts at a distance from the material surface. Fetal rat calvarial cells were plated onto the ceramic and examined by scanning electron microscopy. The findings reported show that cells attached and proliferated on the surface to the ceramic. Attachment by cells to the material can be enhanced by preincubation of psW in serum or media containing fibronectin. The adhesion of cells can be inhibited by addition of GRGDS peptides suggesting that adhesion to psW is mediated by integrin binding to adsorbed proteins. To study the effects of psW at a distance, cells were cultured in the presence but not in direct contact with the material. Subsequent changes in proliferation, alkaline phosphatase expression, and bone nodule formation were assessed. Cells grown in wells containing psW demonstrated an increase in both the rate and total numbers of bone nodules formed, although there were no differences in proliferation or alkaline phosphatase expression. Overall, these results suggest that psW is biocompatible and osteoconductive. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 351–358, 2004
ISSN:1549-3296
0021-9304
1552-4965
1097-4636
DOI:10.1002/jbm.a.30012