Cardiac ankyrin repeat protein, a negative regulator of cardiac gene expression, is augmented in human heart failure

The technique of representational difference analysis of cDNA has been applied to screen for differentially expressed genes in a canine model of pacing-induced heart failure. We identified the canine homolog of the cardiac ankyrin repeat protein (CARP) which has been shown to be involved in the regu...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-05, Vol.293 (5), p.1377-1382
Main Authors: Zolk, Oliver, Frohme, Marcus, Maurer, Alexander, Kluxen, Franz-Werner, Hentsch, Bernd, Zubakov, Dimitri, Hoheisel, Jörg D, Zucker, Irving H, Pepe, Salvatore, Eschenhagen, Thomas
Format: Article
Language:English
Subjects:
Adult
Aged
Animals
Blotting, Northern
Blotting, Western
Cardiac muscle
CARP
Cell Nucleus - metabolism
Dilated cardiomyopathy
DNA, Complementary - metabolism
Dogs
Escherichia coli - metabolism
Female
Gene expression
Gene Expression Regulation
Heart failure
Heart Failure - metabolism
Humans
Ischemic cardiomyopathy
Male
Middle Aged
Muscle Proteins
Myocardium - metabolism
Nuclear Proteins - metabolism
Nucleic Acid Hybridization
Representational difference analysis of cDNA
Repressor Proteins - metabolism
RNA - metabolism
RNA, Messenger - metabolism
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Summary:The technique of representational difference analysis of cDNA has been applied to screen for differentially expressed genes in a canine model of pacing-induced heart failure. We identified the canine homolog of the cardiac ankyrin repeat protein (CARP) which has been shown to be involved in the regulation of the transcription of cardiac genes. To confirm the significance for human heart failure, cardiac tissue specimens obtained from non-failing donor hearts and from explanted hearts from patients with end-stage heart failure were investigated. CARP mRNA and protein levels were markedly increased in failing left ventricles. Interestingly, alterations in CARP expression were restricted to ventricular tissue and were not observed in atria. Fractionation experiments revealed that CARP was expressed predominantly in the nuclei consistent with the proposed function of CARP as a modulator of transcription. Together, these findings raise the possibility that augmented ventricular CARP expression may play a role in the pathogenesis of human heart failure.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)00387-X