3D Structure of Fish Muscle Myosin Filaments

Myosinfilaments isolated from goldfish (Carassius auratus) muscle under relaxing conditions and viewed in negative stain by electron microscopy have been subjected to 3D helical reconstruction to provide details of the myosin head arrangement in relaxed muscle. Previous X-ray diffraction studies of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of structural biology 2002-01, Vol.137 (1-2), p.154-163
Main Authors: Eakins, Felicity, AL-Khayat, Hind A., Kensler, Robert W., Morris, Ed P., Squire, John M.
Format: Article
Language:English
Subjects:
Animals
Computer Simulation
Goldfish
Microscopy, Electron
Models, Biological
Muscles - metabolism
Muscles - ultrastructure
Myosins - chemistry
Protein Structure, Tertiary
X-Ray Diffraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Myosinfilaments isolated from goldfish (Carassius auratus) muscle under relaxing conditions and viewed in negative stain by electron microscopy have been subjected to 3D helical reconstruction to provide details of the myosin head arrangement in relaxed muscle. Previous X-ray diffraction studies of fish muscle (plaice) myosin filaments have suggested that the heads project a long way from the filament surface rather than lying down flat and that heads in a single myosin molecule tend to interact with each other rather than with heads from adjacent molecules. Evidence has also been presented that the head tilt is away from the M-band. Here we seek to confirm these conclusions using a totally independent method. By using 3D helical reconstruction of isolated myosin filaments the known perturbation of the head array in vertebrate muscles was inevitably averaged out. The 3D reconstruction was therefore compared with the X-ray model after it too had been helically averaged. The resulting images showed the same characteristic features: heads projecting out from the filament backbone to high radius and the motor domains at higher radius and further away from the M-band than the light-chain-binding neck domains (lever arms) of the heads.
ISSN:1047-8477
1095-8657
DOI:10.1006/jsbi.2002.4453