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Selected contribution: acute cellular and molecular responses to resistance exercise
Training protocols apply sequential bouts of resistance exercise (RE) to induce the cellular and molecular responses necessary to produce compensatory hypertrophy. This study was designed to 1) define the time course of selected cellular and molecular responses to a single bout of RE and 2) examine...
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Published in: | Journal of applied physiology (1985) 2002-07, Vol.93 (1), p.394-403 |
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creator | Haddad, Fadia Adams, Gregory R |
description | Training protocols apply sequential bouts of resistance exercise (RE) to induce the cellular and molecular responses necessary to produce compensatory hypertrophy. This study was designed to 1) define the time course of selected cellular and molecular responses to a single bout of RE and 2) examine the effects of interbout rest intervals on the summation of these responses. Rat muscles were exposed to RE via stimulation of the sciatic nerve in vivo. Stimulated and control muscles were obtained at various time points post-RE and analyzed via Western blot and RT-PCR. A single bout of RE increased intracellular signaling (i.e., phosphorylations) and expression of mRNAs for insulin-like growth factor-I system components and myogenic markers (e.g., cyclin D1, myogenin). A rest interval of 48 h between RE bouts resulted in much greater summation of myogenic responses than 24- or 8-h rest intervals. This experimental approach should be useful for studying the regulatory mechanisms that control the hypertrophy response. These methods could also be used to compare and contrast different exercise parameters (e.g., concentric vs. eccentric, etc.). |
doi_str_mv | 10.1152/japplphysiol.01153.2001 |
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This study was designed to 1) define the time course of selected cellular and molecular responses to a single bout of RE and 2) examine the effects of interbout rest intervals on the summation of these responses. Rat muscles were exposed to RE via stimulation of the sciatic nerve in vivo. Stimulated and control muscles were obtained at various time points post-RE and analyzed via Western blot and RT-PCR. A single bout of RE increased intracellular signaling (i.e., phosphorylations) and expression of mRNAs for insulin-like growth factor-I system components and myogenic markers (e.g., cyclin D1, myogenin). A rest interval of 48 h between RE bouts resulted in much greater summation of myogenic responses than 24- or 8-h rest intervals. This experimental approach should be useful for studying the regulatory mechanisms that control the hypertrophy response. 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subjects | Animals Biomarkers Cell Differentiation - physiology Cells Exercise Female Functional Laterality - physiology Hypertrophy Insulin - metabolism Insulin - physiology Insulin-Like Growth Factor I - metabolism Insulin-Like Growth Factor I - physiology Molecules Muscle Contraction - physiology Muscle, Skeletal - growth & development Muscle, Skeletal - metabolism Muscle, Skeletal - physiology Organ Size - physiology Phosphorylation Physical Exertion - physiology Protein Sorting Signals - physiology Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA - biosynthesis Signal Transduction - physiology Space life sciences |
title | Selected contribution: acute cellular and molecular responses to resistance exercise |
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