Detection and characterization of OX40 ligand expression in human airway smooth muscle cells: A possible role in asthma?

• Published in: Journal of allergy and clinical immunology 2004-04, Vol.113 (4), p.683-689
• Main Authors: Burgess, Janette K., Carlin, Stephen, Pack, Robert A., Arndt, Greg M., Au, Wendy W., Johnson, Peter R.A., Black, Judith L., Hunt, Nicholas H.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Antigens, Differentiation - pharmacology; Asthma; Asthma - physiopathology; Biological and medical sciences; cell-surface molecules; Cells, Cultured; Chronic obstructive pulmonary disease, asthma; Cytokines; Cytokines - metabolism; Emphysema; Enzyme-Linked Immunosorbent Assay; Female; Flow cytometry; human airway smooth muscle cells; Humans; inflammation; Kinases; Laboratories; Ligands; Lungs; Lymphocytes; Male; Medical sciences; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Middle Aged; Muscle, Smooth - cytology; Muscle, Smooth - drug effects; Muscle, Smooth - metabolism; Muscular system; OX40 Ligand; Patients; Pneumology; Precipitin Tests; Respiratory System - cytology; Respiratory System - drug effects; Respiratory System - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Signal Transduction - drug effects; Smooth muscle
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2003.12.311

The airway smooth muscle (ASM) cell, originally thought of as a passive structural cell, is now well recognized as an active participant in the pathologic events that occur during persistent asthma. Cell-surface molecules play an important role in the development of an immune response. A number of cell-surface molecules are expressed on ASM cells, and these might contribute to the inflammatory reaction. The purpose of this study was to determine whether OX40 ligand (OX40L), a molecule known to be involved in T-cell activation, was present on the ASM cell surface. We used real-time RT-PCR to detect mRNA expression and flow cytometry, ELISA, and immunoprecipitation to detect the presence of cell-surface protein on ASM cells isolated from asthmatic and nonasthmatic individuals. ELISAs and Western blotting were used to determine the functional outcomes of engagement of OX40L. OX40L was present on both asthmatic and nonasthmatic ASM cells. Engagement of OX40L with recombinant OX40:Fc resulted in a significantly greater increase in release of IL-6 from ASM cells of asthmatic patients than from ASM cells of nonasthmatic patients (P