Analysis of chicken TLR4, CD28, MIF, MD-2, and LITAF genes in a Salmonella enteritidis resource population

• Published in: Poultry science 2004-04, Vol.83 (4), p.544-549
• Main Authors: Malek, M, Hasenstein, J.R, Lamont, S.J
• Format: Article
• Language: English
• Subjects: alleles; Animals; antibody formation; breeding lines; cecum; chickens; Chickens - genetics; Chickens - microbiology; disease resistance; Female; Genetic Carrier Screening; genetic markers; genetic resistance; genotype; immune response; inbred lines; line differences; linkage (genetics); lipopolysaccharide-induced tumor necrosis factor; macrophage migration inhibitory factor; macrophages; Male; MD-2 protein; Membrane Glycoproteins - genetics; Nuclear Proteins - genetics; outer membrane proteins; plate count; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; poultry diseases; Poultry Diseases - genetics; Poultry Diseases - immunology; receptors; Receptors, Cell Surface - genetics; Receptors, Immunologic - genetics; Salmonella; Salmonella enteritidis; Salmonella enteritidis - isolation & purification; Salmonella Infections, Animal - genetics; Salmonella Infections, Animal - immunology; single nucleotide polymorphism; spleen; T-lymphocyte surface protein CD28; T-lymphocytes; toll-like receptor; Toll-Like Receptor 4; Toll-Like Receptors; Transcription Factors - genetics; tumor necrosis factors
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1093/ps/83.4.544

Salmonella enteritidis is a foodborne pathogen that negatively affects both animal and human health. Genetic variations in response to pathogenic SE colonization or to SE vaccination were measured in a chicken resource population. Outbred broiler sires and 3 diverse, highly inbred dam lines produced 508 F1 progeny that were evaluated for either bacterial colonization after pathogenic SE inoculation or circulating antibody level after SE vaccination. Five candidate genes were selected for study, based on their biological function as possibly affecting response to SE: toll-like receptor 4 (TLR4), T-cell specific surface protein (CD28), macrophage migration inhibitory factor (MIF), MD-2, and lipopolysaccharide-induced tumor necrosis factor (TNF)-alpha factor (LITAF). Gene fragments were sequenced from the founder lines of the resource population. The LITAF and MIF genes were homozygous for all sires. Single nucleotide polymorphisms (SNP) were identified in 3 genes (TLR4, CD28, and MD-2) and were used to test for associations of sire SNP with SE response. Linear mixed models were used for statistical analyses. The CD28 broiler sire SNP was associated with both bacterial load in the cecum (P < 0.003) and vaccine antibody response (P < 0.05). The MD-2 SNP was associated (P < 0.04) with the bacterial load in the spleen. The use of these SNP in these genes in marker-assisted selection may result in enhancement of disease resistance.