Rab14 is involved in membrane trafficking between the Golgi complex and endosomes

Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. Here, we report the subcellular distribution and function of Rab14. By immunofluorescence and immunoelectron microscopy, both endogenous as well as overexpressed...

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Bibliographic Details
Published in:Molecular biology of the cell 2004-05, Vol.15 (5), p.2218-2229
Main Authors: Junutula, Jagath R, De Maziére, Ann M, Peden, Andrew A, Ervin, Karen E, Advani, Raj J, van Dijk, Suzanne M, Klumperman, Judith, Scheller, Richard H
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Cell Line
Cell Membrane - physiology
Endosomes - physiology
Endosomes - ultrastructure
Gene Expression
Golgi Apparatus - physiology
Golgi Apparatus - ultrastructure
Green Fluorescent Proteins - analysis
Humans
Intracellular Space - ultrastructure
Mice
Microscopy, Fluorescence
Point Mutation - genetics
Protein Transport - physiology
rab GTP-Binding Proteins - analysis
rab GTP-Binding Proteins - genetics
rab GTP-Binding Proteins - physiology
Rats
Receptors, Transferrin - analysis
Receptors, Transferrin - metabolism
Transferrin - metabolism
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Summary:Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. Here, we report the subcellular distribution and function of Rab14. By immunofluorescence and immunoelectron microscopy, both endogenous as well as overexpressed Rab14 were localized to biosynthetic (rough endoplasmic reticulum, Golgi, and trans-Golgi network) and endosomal compartments (early endosomal vacuoles and associated vesicles). Notably overexpression of Rab14Q70L shifted the distribution toward the early endosome associated vesicles, whereas the S25N and N124I mutants induced a shift toward the Golgi region. A similar, although less pronounced, redistribution of the transferrin receptor was also observed in cells overexpressing Rab14 mutants. Impairment of Rab14 function did not however affect transferrin uptake or recycling kinetics. Together, these findings suggest that Rab14 is involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e03-10-0777