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An automated ‘bio-intact’ PTH assay: a step towards standardisation and improved correlation with parathyroid function in renal disease
Background: Most methods for the determination of parathyroid hormone (PTH) show cross-reactivity with N-truncated forms of PTH. The analytic and diagnostic value of a recently developed automated PTH test without this cross-reactivity was examined. Methods: The PTH levels of 73 patients undergoing...
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| Published in: | Clinica chimica acta 2004-05, Vol.343 (1), p.167-171 |
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| container_start_page | 167 |
| container_title | Clinica chimica acta |
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| creator | Savoca, Reto Bock, Andreas Kraenzlin, Marius E. Schmid, Hans-Rudolf Huber, Andreas R. |
| description | Background: Most methods for the determination of parathyroid hormone (PTH) show cross-reactivity with N-truncated forms of PTH. The analytic and diagnostic value of a recently developed automated PTH test without this cross-reactivity was examined.
Methods: The PTH levels of 73 patients undergoing hemodialysis were compared using the ‘bio-intact’ PTH (Nichols Institute Diagnostics) and 3 ‘intact’ PTH tests (from Nichols, Roche Elecsys® and Diagnostics Corporation DPC). Further, the (non 1–84) PTH fragment and the PTH ratio (bio-intact PTH/(non 1–84) PTH) were calculated. All results were then correlated with biochemical bone markers (bone-specific alkaline phosphatase and bone collagen C-terminal telopeptides in serum).
Results: ‘Bio-intact’ PTH values were lower than the PTH results generated by the ‘intact’ PTH assays. Results of all PTH tests were closely correlated (
r=0.96–0.98,
p |
| doi_str_mv | 10.1016/j.cccn.2004.01.024 |
| format | article |
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Methods: The PTH levels of 73 patients undergoing hemodialysis were compared using the ‘bio-intact’ PTH (Nichols Institute Diagnostics) and 3 ‘intact’ PTH tests (from Nichols, Roche Elecsys® and Diagnostics Corporation DPC). Further, the (non 1–84) PTH fragment and the PTH ratio (bio-intact PTH/(non 1–84) PTH) were calculated. All results were then correlated with biochemical bone markers (bone-specific alkaline phosphatase and bone collagen C-terminal telopeptides in serum).
Results: ‘Bio-intact’ PTH values were lower than the PTH results generated by the ‘intact’ PTH assays. Results of all PTH tests were closely correlated (
r=0.96–0.98,
p<0.01). Correlations with biochemical bone markers were high (
r=0.31–0.63,
p<0.01), but no significant association between the PTH ratio and all other tests (
r=−0.2 to 0.03) was found.
Conclusions: In stable hemodialysis patients, the different PTH tests show a similar correlation with the bone markers. It is however desirable to measure PTH with assays devoid of any cross-reaction for a better comparability. In this study, the PTH ratio was not correlated with biochemical bone markers; the use of this ratio requires further investigation.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/j.cccn.2004.01.024</identifier><identifier>PMID: 15115690</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aged ; Automation ; Biomarkers - analysis ; Bone and Bones - metabolism ; Bone markers ; Chemistry, Clinical - standards ; Dialysis ; Female ; Humans ; Intact PTH ; Kidney Diseases - blood ; Kidney Diseases - metabolism ; Male ; Parathyroid Hormone - blood ; Parathyroid Hormone - metabolism ; PTH ; PTH fragments ; Renal Dialysis ; Secondary hyperparathyroidism</subject><ispartof>Clinica chimica acta, 2004-05, Vol.343 (1), p.167-171</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-b98f930dae9cfd7b259b122f1df2d085f667b270c14ae37153950fc58ce761703</citedby><cites>FETCH-LOGICAL-c352t-b98f930dae9cfd7b259b122f1df2d085f667b270c14ae37153950fc58ce761703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15115690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Savoca, Reto</creatorcontrib><creatorcontrib>Bock, Andreas</creatorcontrib><creatorcontrib>Kraenzlin, Marius E.</creatorcontrib><creatorcontrib>Schmid, Hans-Rudolf</creatorcontrib><creatorcontrib>Huber, Andreas R.</creatorcontrib><title>An automated ‘bio-intact’ PTH assay: a step towards standardisation and improved correlation with parathyroid function in renal disease</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Background: Most methods for the determination of parathyroid hormone (PTH) show cross-reactivity with N-truncated forms of PTH. The analytic and diagnostic value of a recently developed automated PTH test without this cross-reactivity was examined.
Methods: The PTH levels of 73 patients undergoing hemodialysis were compared using the ‘bio-intact’ PTH (Nichols Institute Diagnostics) and 3 ‘intact’ PTH tests (from Nichols, Roche Elecsys® and Diagnostics Corporation DPC). Further, the (non 1–84) PTH fragment and the PTH ratio (bio-intact PTH/(non 1–84) PTH) were calculated. All results were then correlated with biochemical bone markers (bone-specific alkaline phosphatase and bone collagen C-terminal telopeptides in serum).
Results: ‘Bio-intact’ PTH values were lower than the PTH results generated by the ‘intact’ PTH assays. Results of all PTH tests were closely correlated (
r=0.96–0.98,
p<0.01). Correlations with biochemical bone markers were high (
r=0.31–0.63,
p<0.01), but no significant association between the PTH ratio and all other tests (
r=−0.2 to 0.03) was found.
Conclusions: In stable hemodialysis patients, the different PTH tests show a similar correlation with the bone markers. It is however desirable to measure PTH with assays devoid of any cross-reaction for a better comparability. In this study, the PTH ratio was not correlated with biochemical bone markers; the use of this ratio requires further investigation.</description><subject>Aged</subject><subject>Automation</subject><subject>Biomarkers - analysis</subject><subject>Bone and Bones - metabolism</subject><subject>Bone markers</subject><subject>Chemistry, Clinical - standards</subject><subject>Dialysis</subject><subject>Female</subject><subject>Humans</subject><subject>Intact PTH</subject><subject>Kidney Diseases - blood</subject><subject>Kidney Diseases - metabolism</subject><subject>Male</subject><subject>Parathyroid Hormone - blood</subject><subject>Parathyroid Hormone - metabolism</subject><subject>PTH</subject><subject>PTH fragments</subject><subject>Renal Dialysis</subject><subject>Secondary hyperparathyroidism</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQhi0EokvhBTggn7gl2E6cxIhLVQFFqgSHcrYm9kT1KrEX22m1t955AXi9PglediVunGbG88-n8fyEvOas5ox377a1McbXgrG2Zrxmon1CNnzom6pplXhKNowxVQ1q4GfkRUrbUras48_JGZecy06xDfl54SmsOSyQ0dLHh1-jC5XzGUx-fPhNv91cUUgJ9u8p0JRxR3O4h2hTKcDbkrkE2YUC8Za6ZRfDXeGYECPOx8a9y7d0BxHy7T4GZ-m0evO34zyN6GGmBYKQ8CV5NsGc8NUpnpPvnz7eXF5V118_f7m8uK5MI0WuRjVMqmEWUJnJ9qOQauRCTNxOwrJBTl1XHntmeAvY9Fw2SrLJyMFg3_GeNefk7ZFbtv2xYsp6ccngPIPHsCbdlxu2sm2KUByFJoaUIk56F90Cca850wcL9FYfLNAHCzTjulhQht6c6Ou4oP03crp5EXw4CrD88c5h1Mk49Aati2iytsH9j_8HtfWb9w</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Savoca, Reto</creator><creator>Bock, Andreas</creator><creator>Kraenzlin, Marius E.</creator><creator>Schmid, Hans-Rudolf</creator><creator>Huber, Andreas R.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>An automated ‘bio-intact’ PTH assay: a step towards standardisation and improved correlation with parathyroid function in renal disease</title><author>Savoca, Reto ; Bock, Andreas ; Kraenzlin, Marius E. ; Schmid, Hans-Rudolf ; Huber, Andreas R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-b98f930dae9cfd7b259b122f1df2d085f667b270c14ae37153950fc58ce761703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aged</topic><topic>Automation</topic><topic>Biomarkers - analysis</topic><topic>Bone and Bones - metabolism</topic><topic>Bone markers</topic><topic>Chemistry, Clinical - standards</topic><topic>Dialysis</topic><topic>Female</topic><topic>Humans</topic><topic>Intact PTH</topic><topic>Kidney Diseases - blood</topic><topic>Kidney Diseases - metabolism</topic><topic>Male</topic><topic>Parathyroid Hormone - blood</topic><topic>Parathyroid Hormone - metabolism</topic><topic>PTH</topic><topic>PTH fragments</topic><topic>Renal Dialysis</topic><topic>Secondary hyperparathyroidism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Savoca, Reto</creatorcontrib><creatorcontrib>Bock, Andreas</creatorcontrib><creatorcontrib>Kraenzlin, Marius E.</creatorcontrib><creatorcontrib>Schmid, Hans-Rudolf</creatorcontrib><creatorcontrib>Huber, Andreas R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Savoca, Reto</au><au>Bock, Andreas</au><au>Kraenzlin, Marius E.</au><au>Schmid, Hans-Rudolf</au><au>Huber, Andreas R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An automated ‘bio-intact’ PTH assay: a step towards standardisation and improved correlation with parathyroid function in renal disease</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>343</volume><issue>1</issue><spage>167</spage><epage>171</epage><pages>167-171</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Background: Most methods for the determination of parathyroid hormone (PTH) show cross-reactivity with N-truncated forms of PTH. The analytic and diagnostic value of a recently developed automated PTH test without this cross-reactivity was examined.
Methods: The PTH levels of 73 patients undergoing hemodialysis were compared using the ‘bio-intact’ PTH (Nichols Institute Diagnostics) and 3 ‘intact’ PTH tests (from Nichols, Roche Elecsys® and Diagnostics Corporation DPC). Further, the (non 1–84) PTH fragment and the PTH ratio (bio-intact PTH/(non 1–84) PTH) were calculated. All results were then correlated with biochemical bone markers (bone-specific alkaline phosphatase and bone collagen C-terminal telopeptides in serum).
Results: ‘Bio-intact’ PTH values were lower than the PTH results generated by the ‘intact’ PTH assays. Results of all PTH tests were closely correlated (
r=0.96–0.98,
p<0.01). Correlations with biochemical bone markers were high (
r=0.31–0.63,
p<0.01), but no significant association between the PTH ratio and all other tests (
r=−0.2 to 0.03) was found.
Conclusions: In stable hemodialysis patients, the different PTH tests show a similar correlation with the bone markers. It is however desirable to measure PTH with assays devoid of any cross-reaction for a better comparability. In this study, the PTH ratio was not correlated with biochemical bone markers; the use of this ratio requires further investigation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15115690</pmid><doi>10.1016/j.cccn.2004.01.024</doi><tpages>5</tpages></addata></record> |
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| subjects | Aged Automation Biomarkers - analysis Bone and Bones - metabolism Bone markers Chemistry, Clinical - standards Dialysis Female Humans Intact PTH Kidney Diseases - blood Kidney Diseases - metabolism Male Parathyroid Hormone - blood Parathyroid Hormone - metabolism PTH PTH fragments Renal Dialysis Secondary hyperparathyroidism |
| title | An automated ‘bio-intact’ PTH assay: a step towards standardisation and improved correlation with parathyroid function in renal disease |
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