Comparative Analysis of Commercial Assays for the Detection and Quantification of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Plasma from Patients Infected with HIV-1 Subtype C

The commercial assays commonly used to quantify plasma human immunodeficiency virus type 1 (HIV-1) RNA in clinical settings were designed to assess HIV-1 subtype B. We compared the performance of 4 commercial assays (Amplicor versions 1.0 and 1.5 [Roche]; Quantiplex [Chiron]; and NASBA HIV-1 RNA QT...

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Bibliographic Details
Published in:Clinical infectious diseases 2002-08, Vol.35 (3), p.323-325
Main Authors: Hoesley, Craig J., Allen, Susan A., Raper, James L., Musonda, R., Niu, Yafen, Gao, Feng, Squires, Kathleen E., Aldrovandi, Grace M.
Format: Article
Language:English
Subjects:
AIDS
Biological and medical sciences
Blood plasma
Female
HIV 1
HIV Infections - blood
HIV-1 - genetics
HIV-1 - physiology
HIV/AIDS
Human viral diseases
Humans
Infections
Infectious diseases
Male
Medical genetics
Medical sciences
Quantification
RNA
RNA, Viral - blood
Self sustained sequence replication
Specimens
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Load
Viruses
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Summary:The commercial assays commonly used to quantify plasma human immunodeficiency virus type 1 (HIV-1) RNA in clinical settings were designed to assess HIV-1 subtype B. We compared the performance of 4 commercial assays (Amplicor versions 1.0 and 1.5 [Roche]; Quantiplex [Chiron]; and NASBA HIV-1 RNA QT [Organon Teknika]) in detecting and quantifying HIV-1 RNA in plasma from HIV-infected persons from Zambia, an area where HIV-1 subtype C is predominant. Each assay detected plasma HIV-1 RNA, but they do not all measure statistically similar quantities of plasma HIV-1 RNA.>
ISSN:1058-4838
1537-6591
DOI:10.1086/341490