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Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O157:H7
Sera from cattle naturally infected with Brucella abortus ( n=160), vaccinated with B. abortus S19 ( n=88) or immunized with Yersinia enterocolitica O:9 ( n=25) or Escherichia coli O157:H7 ( n=80) were collected. The sera were compared for antibody content to the same bacteria by indirect enzyme imm...
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Published in: | Veterinary microbiology 2004-05, Vol.100 (1), p.25-30 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | Sera from cattle naturally infected with
Brucella abortus (
n=160), vaccinated with
B. abortus S19 (
n=88) or immunized with
Yersinia enterocolitica O:9 (
n=25) or
Escherichia coli O157:H7 (
n=80) were collected. The sera were compared for antibody content to the same bacteria by indirect enzyme immunoassay (IELISA), fluorescence polarization assay (FPA) and competitive enzyme immunoassay (CELISA). Cattle sera (
n=523) collected randomly from across Canada were tested in the same tests.
Sera from the
B. abortus infected group reacted positively in the brucellosis IELISA (IELISA
Br), CELISA and FPA (FPA
Br) and the
Y. enterocolitica IELISA (IELISA
Ye) while the
Y. enterocolitica FPA (FPA
Ye) detected antibody in 93.8% and the
E. coli IELISA (IELISA
Ec) 86.9% and the
E. coli FPA (FPA
Ec) 48.1%. About 70% of the sera from
B. abortus S19 vaccinated animals reacted in the three IELISAs, 45% in the CELISA, and 37.7% in the FPA
Ec, 21.6% in the FPA
Br and 5.7% in the FPA
Ye. Sera from
E. coli O:157 exposed cattle reacted mainly in the IELISA
Ec and FPA
Ec although surprisingly 87.5% reacted in the IELISA
Ye and only 3.8% in the IELISA
Br. No reactions were observed with these sera in the FPA
Br and FPA
Ye but one serum gave a low positive reaction in the CELISA. All sera from
Y. enterocolitica O:9 exposed cattle reacted in the IELISA
Br and IELISA
Ye and 80% in the IELISA
Ec. In the CELISA, 44% gave a positive reaction and 64% were positive in the FPA
Br, 28% in the FPA
Ye and 12% in the FPA
Ec. Of the 523 Canadian sera, about 50% reacted in the
E. coli tests with only minor reactions in the
Y. enterocolitica O:9 and
B. abortus assays.
From the data, the cross reaction between
E. coli O157:H7,
Y. enterocilitica O:9 and
B. abortus is dependent on the test used. Thus, extensive cross reaction was observed with the IELISA with much less reactivity in the FPA and the CELISA. |
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ISSN: | 0378-1135 1873-2542 |
DOI: | 10.1016/j.vetmic.2003.12.010 |