Duckweed ( Lemna polyrhiza) leaf meal as a source of feedstuff in formulated diets for rohu ( Labeo rohita Ham.) fingerlings after fermentation with a fish intestinal bacterium

• Published in: Bioresource technology 2002-10, Vol.85 (1), p.17-24
• Main Authors: Bairagi, A, Sarkar Ghosh, K, Sen, S.K, Ray, A.K
• Format: Article
• Language: English
• Subjects: Animal aquaculture; Animal Feed; Animal productions; Animals; antinutritional factors; Araceae; Bacillus (bacteria); Bacillus - enzymology; Bacillus - isolation & purification; bacteria; Biological and medical sciences; Body Weight; carp; Carps - growth & development; Carps - physiology; crude protein; Cyprinus carpio; Diets; digestible protein; Digestion - drug effects; experimental diets; fatty acids; Feed and pet food industries; feed conversion; feeds; Fermentation; fiber content; fingerlings; fish meal; food conversion; Food industries; free amino acids; Fundamental and applied biological sciences. Psychology; Growth performance; Intestinal bacteria; intestines; Intestines - microbiology; Labeo rohita; Labeo rohita fingerlings; leaves; Lemna; Lemna polyrhiza; Lipids - analysis; phytic acid; Pisciculture; Plant Leaves - metabolism; protein efficiency ratio; Proteins - analysis; proteolysis; reducing sugars; Reference Values; Spirodela polyrhiza; Time Factors; Vertebrate aquaculture
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/S0960-8524(02)00067-6

Eight isonitrogenous (35% crude protein approximately) and isocaloric (4.2 kcal g −1 approximately) diets were formulated including raw and fermented duckweed ( Lemna polyrhiza) leaf meal at 10%, 20%, 30% and 40% levels. A particular bacterial strain ( Bacillus sp.) isolated from carp ( Cyprinus carpio) intestine and having extracellular amylolytic, cellulolytic, proteolytic and lipolytic activities was used for leaf meal fermentation for 15 days at 37 °C. The fibre content of leaf meal reduced from 11.0% to 7.5% and the antinutritional factors, tannin and phytic acid, were reduced from 1.0% to 0.02% and 1.23% to 0.09%, respectively after fermentation. However, the available reducing sugars, free amino acids and fatty acids increased in the fermented leaf meal. The response of rohu, Labeo rohita, fingerlings fed the experimental diets for 80 days was compared with fish fed a fish meal based reference diet. On the basis of growth response, food conversion ratio and protein efficiency ratio, 30% fermented Lemna leaf meal incorporated in the diet resulted in the best performance of rohu fingerlings. In general, growth and feed utilization efficiencies of fish fed fermented leaf meal containing diets were superior to those fed diets containing raw leaf meal. The apparent protein digestibility (APD) decreased with increasing levels of leaf meal irrespective of treatment. The APD for raw leaf meal was lower at all levels of inclusion in comparison to those for the fermented meals. The highest carcass protein and lipid deposition was recorded in fish fed the diet containing 30% fermented leaf meal. The results showed that fermented Lemna leaf meal can be incorporated into carp diets up to 30% level compared to 10% level of raw meal.