Assaying the DNA damage checkpoint in fission yeast

Cell cycle checkpoints exist to ensure the proper maintenance and stable inheritance of genomic information. The pathways that insure the faithful execution of these checkpoints are well conserved throughout evolution. In the fission yeast, Schizosaccharomyces pombe, a major cell cycle checkpoint ex...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2004-07, Vol.33 (3), p.260-263
Main Authors: Dunaway, Stephen, Walworth, Nancy C.
Format: Article
Language:English
Subjects:
DNA Damage - physiology
DNA, Fungal - analysis
DNA, Fungal - metabolism
Schizosaccharomyces - chemistry
Schizosaccharomyces - metabolism
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Summary:Cell cycle checkpoints exist to ensure the proper maintenance and stable inheritance of genomic information. The pathways that insure the faithful execution of these checkpoints are well conserved throughout evolution. In the fission yeast, Schizosaccharomyces pombe, a major cell cycle checkpoint exists that responds to the presence of damaged DNA and prevents this damage from being propagated to future generations. Fission yeast is an ideal system to investigate these pathways because there exist specific techniques that allow one to assay the fidelity of this DNA damage checkpoint pathway.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2003.11.022