Genetic analysis of hepatitis A virus isolates from Brazil

A limited number of hepatitis A virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti‐HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were...

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Bibliographic Details
Published in:Journal of medical virology 2004-07, Vol.73 (3), p.378-383
Main Authors: de Paula, Vanessa S., Lu, Ling, Niel, Christian, Gaspar, Ana M.C., Robertson, Betty H.
Format: Article
Language:English
Subjects:
acute hepatitis
Adolescent
Adult
Aged
Amino Acid Substitution
Biological and medical sciences
Brazil
Child
Child, Preschool
Female
Fundamental and applied biological sciences. Psychology
Genetics
Genome, Viral
Genotype
Hepatitis A - virology
Hepatitis A virus
Hepatitis A virus - classification
Hepatitis A virus - genetics
Hepatitis A virus - isolation & purification
Human viral diseases
Humans
Infectious diseases
Male
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Molecular Epidemiology
Molecular Sequence Data
nucleotide sequencing
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - blood
RNA, Viral - genetics
RNA, Viral - isolation & purification
Sequence Analysis, DNA
Sequence Homology
subgenotype IA
subgenotype IB
Viral diseases
Viral Structural Proteins - chemistry
Viral Structural Proteins - genetics
Virology
VP1/2A junction
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Summary:A limited number of hepatitis A virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti‐HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were used to obtain HAV isolates and determine their genetic relatedness. Of the 232 case isolates, sequence data were obtained from the VP1/2A junction region of the HAV genome. All isolates were classified in genotype I; 231 belonged to subgenotype IA, and one to subgenotype IB. HAV isolates from four States formed distinct clusters of highly related sequences. However, isolates from other states did not cluster and the sequences from those states were intermingled with sequences found in the other states. The amino acid sequences of all but two isolates showed a Leu → Ile substitution at position 42 in the 2A protein. This substitution appeared to be a characteristic geographic fingerprint of HAV sequences within Brazil. J. Med. Virol. 73:378–383, 2004. © 2004 Wiley‐Liss, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.20101