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Genetic analysis of hepatitis A virus isolates from Brazil
A limited number of hepatitis A virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti‐HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were...
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| Published in: | Journal of medical virology 2004-07, Vol.73 (3), p.378-383 |
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| cites | cdi_FETCH-LOGICAL-c4861-49490932d766e3df671d01a713af9a810e9ea40957709ba71db7a06fa9b05b7a3 |
| container_end_page | 383 |
| container_issue | 3 |
| container_start_page | 378 |
| container_title | Journal of medical virology |
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| creator | de Paula, Vanessa S. Lu, Ling Niel, Christian Gaspar, Ana M.C. Robertson, Betty H. |
| description | A limited number of hepatitis A virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti‐HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were used to obtain HAV isolates and determine their genetic relatedness. Of the 232 case isolates, sequence data were obtained from the VP1/2A junction region of the HAV genome. All isolates were classified in genotype I; 231 belonged to subgenotype IA, and one to subgenotype IB. HAV isolates from four States formed distinct clusters of highly related sequences. However, isolates from other states did not cluster and the sequences from those states were intermingled with sequences found in the other states. The amino acid sequences of all but two isolates showed a Leu → Ile substitution at position 42 in the 2A protein. This substitution appeared to be a characteristic geographic fingerprint of HAV sequences within Brazil. J. Med. Virol. 73:378–383, 2004. © 2004 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jmv.20101 |
| format | article |
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IgM anti‐HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were used to obtain HAV isolates and determine their genetic relatedness. Of the 232 case isolates, sequence data were obtained from the VP1/2A junction region of the HAV genome. All isolates were classified in genotype I; 231 belonged to subgenotype IA, and one to subgenotype IB. HAV isolates from four States formed distinct clusters of highly related sequences. However, isolates from other states did not cluster and the sequences from those states were intermingled with sequences found in the other states. The amino acid sequences of all but two isolates showed a Leu → Ile substitution at position 42 in the 2A protein. This substitution appeared to be a characteristic geographic fingerprint of HAV sequences within Brazil. J. Med. Virol. 73:378–383, 2004. © 2004 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.20101</identifier><identifier>PMID: 15170631</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>acute hepatitis ; Adolescent ; Adult ; Aged ; Amino Acid Substitution ; Biological and medical sciences ; Brazil ; Child ; Child, Preschool ; Female ; Fundamental and applied biological sciences. Psychology ; Genetics ; Genome, Viral ; Genotype ; Hepatitis A - virology ; Hepatitis A virus ; Hepatitis A virus - classification ; Hepatitis A virus - genetics ; Hepatitis A virus - isolation & purification ; Human viral diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; Molecular Epidemiology ; Molecular Sequence Data ; nucleotide sequencing ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - blood ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; Sequence Analysis, DNA ; Sequence Homology ; subgenotype IA ; subgenotype IB ; Viral diseases ; Viral Structural Proteins - chemistry ; Viral Structural Proteins - genetics ; Virology ; VP1/2A junction</subject><ispartof>Journal of medical virology, 2004-07, Vol.73 (3), p.378-383</ispartof><rights>Copyright © 2004 Wiley‐Liss, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4861-49490932d766e3df671d01a713af9a810e9ea40957709ba71db7a06fa9b05b7a3</citedby><cites>FETCH-LOGICAL-c4861-49490932d766e3df671d01a713af9a810e9ea40957709ba71db7a06fa9b05b7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15778782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15170631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Paula, Vanessa S.</creatorcontrib><creatorcontrib>Lu, Ling</creatorcontrib><creatorcontrib>Niel, Christian</creatorcontrib><creatorcontrib>Gaspar, Ana M.C.</creatorcontrib><creatorcontrib>Robertson, Betty H.</creatorcontrib><title>Genetic analysis of hepatitis A virus isolates from Brazil</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>A limited number of hepatitis A virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti‐HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were used to obtain HAV isolates and determine their genetic relatedness. Of the 232 case isolates, sequence data were obtained from the VP1/2A junction region of the HAV genome. All isolates were classified in genotype I; 231 belonged to subgenotype IA, and one to subgenotype IB. HAV isolates from four States formed distinct clusters of highly related sequences. However, isolates from other states did not cluster and the sequences from those states were intermingled with sequences found in the other states. The amino acid sequences of all but two isolates showed a Leu → Ile substitution at position 42 in the 2A protein. This substitution appeared to be a characteristic geographic fingerprint of HAV sequences within Brazil. J. Med. Virol. 73:378–383, 2004. © 2004 Wiley‐Liss, Inc.</description><subject>acute hepatitis</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Substitution</subject><subject>Biological and medical sciences</subject><subject>Brazil</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Genome, Viral</subject><subject>Genotype</subject><subject>Hepatitis A - virology</subject><subject>Hepatitis A virus</subject><subject>Hepatitis A virus - classification</subject><subject>Hepatitis A virus - genetics</subject><subject>Hepatitis A virus - isolation & purification</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequencing</subject><subject>Phylogeny</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - blood</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>subgenotype IA</subject><subject>subgenotype IB</subject><subject>Viral diseases</subject><subject>Viral Structural Proteins - chemistry</subject><subject>Viral Structural Proteins - genetics</subject><subject>Virology</subject><subject>VP1/2A junction</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqF0E1P20AQBuBVRVVC6KF_APlCJQ5OZvyx4-UWohKKKJUqmnJbTey1WGrHYdcB0l-PIQF6QZxmZ_XMjPQK8QVhgADR8Lq-HUSAgB9ED0HJUAHhlugBJjKUEtNtseP9NQBkKoo-iW1MkUDG2BOHEzM3rc0DnnO18tYHTRlcmQW3tu2aUXBr3dIH1jcVt8YHpWvq4MjxP1vtio8lV9583tS--H387WJ8Ep79nHwfj87CPMkkholKFKg4KkhKExelJCwAmTDmUnGGYJThBFRKBGrW_RczYpAlqxmk3TPui6_rvQvX3CyNb3VtfW6qiuemWXpNqGSSZvJdiKQoSyHu4MEa5q7x3plSL5yt2a00gn5MVHeJ6qdEO7u3Wbqc1aZ4lZsIO7C_AexzrkrH89z6_xxRRlnUueHa3dnKrN6-qE9_TJ9Ph-sJ61tz_zLB7q-WFFOq_5xPdDa-vPx1TFM9iR8A8kWaCQ</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>de Paula, Vanessa S.</creator><creator>Lu, Ling</creator><creator>Niel, Christian</creator><creator>Gaspar, Ana M.C.</creator><creator>Robertson, Betty H.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>Genetic analysis of hepatitis A virus isolates from Brazil</title><author>de Paula, Vanessa S. ; Lu, Ling ; Niel, Christian ; Gaspar, Ana M.C. ; Robertson, Betty H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4861-49490932d766e3df671d01a713af9a810e9ea40957709ba71db7a06fa9b05b7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>acute hepatitis</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Substitution</topic><topic>Biological and medical sciences</topic><topic>Brazil</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Genome, Viral</topic><topic>Genotype</topic><topic>Hepatitis A - virology</topic><topic>Hepatitis A virus</topic><topic>Hepatitis A virus - classification</topic><topic>Hepatitis A virus - genetics</topic><topic>Hepatitis A virus - isolation & purification</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequencing</topic><topic>Phylogeny</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - blood</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>subgenotype IA</topic><topic>subgenotype IB</topic><topic>Viral diseases</topic><topic>Viral Structural Proteins - chemistry</topic><topic>Viral Structural Proteins - genetics</topic><topic>Virology</topic><topic>VP1/2A junction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Paula, Vanessa S.</creatorcontrib><creatorcontrib>Lu, Ling</creatorcontrib><creatorcontrib>Niel, Christian</creatorcontrib><creatorcontrib>Gaspar, Ana M.C.</creatorcontrib><creatorcontrib>Robertson, Betty H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Paula, Vanessa S.</au><au>Lu, Ling</au><au>Niel, Christian</au><au>Gaspar, Ana M.C.</au><au>Robertson, Betty H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of hepatitis A virus isolates from Brazil</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2004-07</date><risdate>2004</risdate><volume>73</volume><issue>3</issue><spage>378</spage><epage>383</epage><pages>378-383</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>A limited number of hepatitis A virus (HAV) isolates from South America have been characterised at the genomic level. IgM anti‐HAV positive serum samples collected from patients with hepatitis A living in the five geographical regions of Brazil (North, Northeast, Central, South, and Southeast) were used to obtain HAV isolates and determine their genetic relatedness. Of the 232 case isolates, sequence data were obtained from the VP1/2A junction region of the HAV genome. All isolates were classified in genotype I; 231 belonged to subgenotype IA, and one to subgenotype IB. HAV isolates from four States formed distinct clusters of highly related sequences. However, isolates from other states did not cluster and the sequences from those states were intermingled with sequences found in the other states. The amino acid sequences of all but two isolates showed a Leu → Ile substitution at position 42 in the 2A protein. This substitution appeared to be a characteristic geographic fingerprint of HAV sequences within Brazil. J. Med. Virol. 73:378–383, 2004. © 2004 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15170631</pmid><doi>10.1002/jmv.20101</doi><tpages>6</tpages></addata></record> |
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| subjects | acute hepatitis Adolescent Adult Aged Amino Acid Substitution Biological and medical sciences Brazil Child Child, Preschool Female Fundamental and applied biological sciences. Psychology Genetics Genome, Viral Genotype Hepatitis A - virology Hepatitis A virus Hepatitis A virus - classification Hepatitis A virus - genetics Hepatitis A virus - isolation & purification Human viral diseases Humans Infectious diseases Male Medical sciences Microbiology Middle Aged Miscellaneous Molecular Epidemiology Molecular Sequence Data nucleotide sequencing Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - blood RNA, Viral - genetics RNA, Viral - isolation & purification Sequence Analysis, DNA Sequence Homology subgenotype IA subgenotype IB Viral diseases Viral Structural Proteins - chemistry Viral Structural Proteins - genetics Virology VP1/2A junction |
| title | Genetic analysis of hepatitis A virus isolates from Brazil |
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