Immunolocalization of TASK-3 (KCNK9) to a subset of cortical neurons in the rat CNS

Tandem pore domain (2P) K channels constitute the most diverse family of K channels and are responsible for background (leak or baseline) K currents. Of the 15 human 2P K channels, TASK-1, TASK-2, and TASK-3 are uniquely sensitive to physiologic pH changes as well as being inhibited by local anesthe...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-06, Vol.319 (2), p.525-530
Main Authors: Callahan, Robert, Labunskiy, Dmitriy A, Logvinova, Anna, Abdallah, Mona, Liu, Canhui, Cotten, Joseph F, Yost, C.Spencer
Format: Article
Language:English
Subjects:
Animals
Background potassium channels
Cerebellum
Cerebral Cortex - cytology
Cerebral Cortex - metabolism
Cerebral Cortex - ultrastructure
Cortex
Hippocampus
Hydrogen-Ion Concentration
Immunohistochemistry
Male
Microscopy, Electron
Neurons - metabolism
Neurons - ultrastructure
Potassium Channels - metabolism
Potassium Channels, Tandem Pore Domain
Rat
Rats
Rats, Sprague-Dawley
Subcellular Fractions - metabolism
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Summary:Tandem pore domain (2P) K channels constitute the most diverse family of K channels and are responsible for background (leak or baseline) K currents. Of the 15 human 2P K channels, TASK-1, TASK-2, and TASK-3 are uniquely sensitive to physiologic pH changes as well as being inhibited by local anesthetics and activated by volatile anesthetics. In this study polyclonal antibodies selective for TASK-3 have been used to localize its expression in the rat central nervous system (CNS). TASK-3 immunostaining was found in rat cortex, hypothalamus, and hippocampus. Double immunofluorescent studies identified a discrete population of TASK-3 expressing neurons scattered throughout cortex. Using immunogold electron microscopy TASK-3 was identified at the cell surface associated with synapses and within the intracellular synthetic compartments. These results provide a more finely detailed picture of TASK-3 expression and indicate a role for TASK-3 in modulating cerebral synaptic transmission and responses to CNS active drugs.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.05.023