Accumulation of 8-nitroguanine in human gastric epithelium induced by Helicobacter pylori infection

Helicobacter pylori infection causes chronic inflammation, which can lead to gastric carcinoma. A double immunofluorescence labeling study demonstrated that the level of 8-nitroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) apparent in gastric gland epithelium was significantly higher in...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-06, Vol.319 (2), p.506-510
Main Authors: Ma, Ning, Adachi, Yukihiko, Hiraku, Yusuke, Horiki, Noriyuki, Horiike, Shinichirou, Imoto, Ichiro, Pinlaor, Somchai, Murata, Mariko, Semba, Reiji, Kawanishi, Shosuke
Format: Article
Language:English
Subjects:
8-Nitroguanine
8-Oxo-7,8-dihydro-2′-deoxyguanosine
Adult
Aged
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - metabolism
DNA damage
Double immunofluorescence labeling
Female
Gastric cancer
Gastric gland epithelial cell
Gastric Mucosa - metabolism
Gastric Mucosa - microbiology
Guanine - analogs & derivatives
Guanine - metabolism
Helicobacter Infections - metabolism
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - isolation & purification
Humans
Infection
Inflammation
Male
Middle Aged
Proliferating cell nuclear antigen
Proliferating Cell Nuclear Antigen - metabolism
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Summary:Helicobacter pylori infection causes chronic inflammation, which can lead to gastric carcinoma. A double immunofluorescence labeling study demonstrated that the level of 8-nitroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) apparent in gastric gland epithelium was significantly higher in gastritis patients with H. pylori infection than in those without infection. A significant accumulation of proliferating cell nuclear antigen, a prognostic factor for gastric cancer, was observed in gastric gland epithelial cells in patients with H. pylori infection as compared to those without infection, and its accumulation was closely correlated with the formation of 8-nitroguanine and 8-oxodG. These results suggest that nitrosative and oxidative DNA damage in gastric epithelial cells and their proliferation by H. pylori infection may lead to gastric carcinoma. 8-Nitroguanine could be not only a promising biomarker for inflammation but also a useful indicator of the risk of gastric cancer development in response to chronic H. pylori infection.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.04.193