Total extracellular surfactant is increased but abnormal in a rat model of gram-negative bacterial pneumonia

Departments of 1  Medicine and 2  Microbiology, The Centers for 3  Microbial Pathogenesis, 4  Pediatrics, and 5  Anesthesiology, State University of New York at Buffalo, Buffalo 14214; 7  Department of Pediatrics, University of Rochester, Rochester, New York 14642; and 6  Department of Medicine, Uni...

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Published in:American journal of physiology. Lung cellular and molecular physiology 2002-09, Vol.283 (3), p.655-L663
Main Authors: Russo, Thomas A, Bartholomew, Lori A, Davidson, Bruce A, Helinski, Jadwiga D, Carlino, Ulrike B, Knight, Paul R., III, Beers, Michael F, Atochina, Elena N, Notter, Robert H, Holm, Bruce A
Format: Article
Language:English
Subjects:
Animals
Bronchoalveolar Lavage Fluid - chemistry
Colony Count, Microbial
Escherichia coli - physiology
Extracellular Space - metabolism
Gram-Negative Bacterial Infections
Phospholipids - analysis
Pneumonia, Bacterial - metabolism
Pneumonia, Bacterial - microbiology
Pneumonia, Bacterial - physiopathology
Proteins - analysis
Pulmonary Surfactants - metabolism
Rats
Rats, Long-Evans
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Summary:Departments of 1  Medicine and 2  Microbiology, The Centers for 3  Microbial Pathogenesis, 4  Pediatrics, and 5  Anesthesiology, State University of New York at Buffalo, Buffalo 14214; 7  Department of Pediatrics, University of Rochester, Rochester, New York 14642; and 6  Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 An in vivo rat model was used to evaluate the effects of Escherichia coli pneumonia on lung function and surfactant in bronchoalveolar lavage (BAL). Total extracellular surfactant was increased in infected rats compared with controls. BAL phospholipid content in infected rats correlated with the severity of alveolar-capillary leak as reflected in lavage protein levels ( R 2  = 0.908, P 50 mg total BAL protein. There was a significant correlation ( R 2  = 0.885, P 50 mg of total BAL protein, consistent with surfactant dysfunction. In vitro surface cycling studies with calf lung surfactant extract suggested that bacterially derived factors may have contributed in part to the surfactant alterations seen in vivo. Escherichia coli ; pulmonary injury; phospholipid; surfactant dysfunction
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00071.2002