Could the coculture of skeletal myoblasts and mesenchymal stem cells be a solution for postinfarction myocardial scar?

• Published in: Transplantation proceedings 2004-05, Vol.36 (4), p.991-992
• Main Authors: Carvalho, K.A.T, Guarita-Souza, L.C, Rebelatto, C.L.K, Senegaglia, A.C, Hansen, P, Mendonça, J.G.R, Cury, C.C, Francisco, J.C, Brofman, P.R.S
• Format: Article
• Language: English
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Applied cell therapy and gene therapy; Biological and medical sciences; Coculture Techniques; Disease Models, Animal; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Heart Transplantation; Medical sciences; Mesoderm - cytology; Muscle, Skeletal - cytology; Myoblasts - cytology; Postoperative Complications - therapy; Rats; Stem Cell Transplantation; Stem Cells - cytology; Tissue, organ and graft immunology; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2004.03.056

Currently two lines of research have been proposed for treatment of heart failure in an attempt to address its main cause: skeletal myoblast (SM) transplants, which increase the contractile muscular mass, and mesenchymal stem cell (MSC) transplants, which increase neoangiogenisis. The objective of this study was to establish methods whereby cocultures of SM and MSC proliferate and expand, making possible the interaction of these cell types prior to their transplantation to the myocardium. Seeking to support the survival of these cells after myocardial transplantation and achieve subsequent functional improvement, SM and MSC from 10 rats were isolated and cultivated in DMEM medium supplemented with 15% fetal calf serum, 1% ATB, and growth factors. Following plating in variable proportions of satellite cells/mononuclear cells namely 2:1, 1:1, 1:2, morphological observations were made regarding cell survival, adhesion to substrate, and confluence. After 48 hours nonadherent cells were aspirated from the flasks, leaving the adherent cells, SM, and MSC. The better level of cell proliferation was observed with the proportion 2:1 cocultivated at a concentration of 5 × 10 5/mL for 14 days. The results were satisfactory; the cell production was up to 10 8, increasing the chances of transplant success after myocardial infarction. Transplants with this model are ongoing.