Distinct Response of Human B Cell Subpopulations in Recognition of an Innate Immune Signal, CpG DNA

Innate immunity has recently gained renewed interest in its ability to regulate adaptive immunity. Among the innate immune signals, CpG DNA has revealed its potential as a vaccine adjuvant. However, the cellular mechanism for the effect of CpG DNA on the humoral immune response is not well understoo...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 2002-09, Vol.169 (5), p.2368-2373
Main Authors: Jung, Jaeho, Yi, Ae-Kyung, Zhang, Xin, Choe, Jongseon, Li, Li, Choi, Yong Sung
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
B-Lymphocyte Subsets - cytology
B-Lymphocyte Subsets - immunology
Cell Division - immunology
Cell Line
CpG Islands - immunology
DNA - immunology
DNA - pharmacology
DNA, Bacterial - pharmacology
Escherichia coli - genetics
Escherichia coli - immunology
Germinal Center - cytology
Germinal Center - immunology
Humans
Immunity, Innate - immunology
Immunologic Memory - immunology
Interphase - immunology
Lymphocyte Activation - immunology
Oligodeoxyribonucleotides - immunology
Oligodeoxyribonucleotides - pharmacology
Plasma Cells - cytology
Plasma Cells - immunology
Signal Transduction - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Innate immunity has recently gained renewed interest in its ability to regulate adaptive immunity. Among the innate immune signals, CpG DNA has revealed its potential as a vaccine adjuvant. However, the cellular mechanism for the effect of CpG DNA on the humoral immune response is not well understood. Here, we investigated the effects of CpG DNA on human B cell differentiation using highly purified B cell subsets: naive, germinal center (GC), and memory B cells. In the in vitro culture system that mimics the primary or secondary immune response in vivo, CpG DNA markedly augmented the proliferation and generation of plasma cells from naive and memory B cells. CpG DNA dramatically increased plasma cell generation from GC B cells. However, CpG DNA did not have effect on memory B cell generation from GC B cells. These results suggest that CpG DNA potentiates the B cell adaptive immune response by enhancing terminal differentiation, but does not affect the generation of memory B cells.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.169.5.2368