A role for ATP hydrolysis in vaccinia virus early gene transcription. Dissociation of the early transcription factor-promoter complex

Vaccinia virus RNA polymerase requires the vaccinia early transcription factor, VETF, for the in vitro initiation of transcription at early gene promoters in a reaction requiring ATP hydrolysis. VETF binds specifically to early gene promoters and has an associated DNA-dependent ATPase activity. The...

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Bibliographic Details
Published in:The Journal of biological chemistry 1991-08, Vol.266 (23), p.15545-15548
Main Author: BROYLES, S. S
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Base Sequence
Biological and medical sciences
DNA, Viral - genetics
DNA, Viral - metabolism
Fundamental and applied biological sciences. Psychology
Genes, Viral
Hydrolysis
Kinetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Promoter Regions, Genetic
Transcription Factors - metabolism
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
vaccinia virus
Vaccinia virus - genetics
Viral Proteins
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Summary:Vaccinia virus RNA polymerase requires the vaccinia early transcription factor, VETF, for the in vitro initiation of transcription at early gene promoters in a reaction requiring ATP hydrolysis. VETF binds specifically to early gene promoters and has an associated DNA-dependent ATPase activity. The effect of ATP on the interaction of VETF with the promoter for the vaccinia growth factor gene promoter has been examined. ATP had no marked effect on the steady-state level of promoter binding but dramatically affected the kinetics of dissociation of VETF from the promoter. The half-life of the VETF-promoter complex was greatly reduced in the presence of ATP. The destabilization of the complex was specific for ATP and dATP, consistent with the substrate specificity of the VETF-associated ATPase. ADP or the non-hydrolyzable ATP analog adenylyl-imidodiphosphate did not destabilize the complex suggesting that ATP hydrolysis is obligatory for dissociation. These findings provide a link between the promoter binding and ATPase activities associated with VETF and suggest that the ATP-dependent dissociation of the VETF-promoter complex is an important event in the transcription of vaccinia virus early genes.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)98651-5