Peripheral Blood Mononuclear Cell Proliferation to Heat Shock Protein-70 Derived from Autologous Lung Carcinoma

In animals, tumor-derived heat shock proteins (HSP) induce immune-mediated protection against autologous cancer. We investigated whether HSP-70 derived from human lung carcinoma are also complexed to tumor-specific antigens. Peripheral blood mononuclear cells collected 10 days after surgery from pat...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2002-09, Vol.166 (5), p.749-753
Main Authors: Michils, Alain, Dutry, Dominique, de Beyl, Valerie Zegers, Remmelink, Myriam, de Maertelaer, Viviane, Rocmans, Pierre
Format: Article
Language:English
Subjects:
Adenocarcinoma - pathology
Aged
Analysis of Variance
Biological and medical sciences
Carcinoma, Squamous Cell - pathology
Cell Division - drug effects
Female
HSP70 Heat-Shock Proteins - pharmacology
Humans
Immunoblotting
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - physiology
Lung Neoplasms - pathology
Male
Medical sciences
Middle Aged
Pneumology
Probability
Sensitivity and Specificity
Transplantation, Autologous
Tumor Cells, Cultured
Tumors of the respiratory system and mediastinum
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Summary:In animals, tumor-derived heat shock proteins (HSP) induce immune-mediated protection against autologous cancer. We investigated whether HSP-70 derived from human lung carcinoma are also complexed to tumor-specific antigens. Peripheral blood mononuclear cells collected 10 days after surgery from patients with lung cancer were stimulated with HSP-70 purified from autologous and heterologous tumors. The stimulation index (SI) obtained when stimulating cells with autologous tumor-derived HSP-70 averaged 3.07 +/- 0.75 in patients with lung cancer and 1.57 +/- 0.33 in control subjects (p < 0.001 by analysis of variance). No significant stimulation was observed with HSP-70 derived either from the majority of heterologous tumors or from autologous tumor-free lung tissue. SI decreased from 3.59 +/- 0.65 to 1.65 +/- 0.38 in six patients tested again 3 months after surgery (p = 0.02 by Wilcoxon test for paired data). HSP-70 derived from lung carcinoma are shown to be associated with T cell antigens. The T cell reactivity appears transient and restricted to antigens complexed to HSP-70 derived from autologous tumors only. This suggests that the antigenicity of human lung tumors is unique, which may be crucial for the design of new vaccines.
ISSN:1073-449X
1535-4970
DOI:10.1164/rccm.200202-126OC