In vivo GABA+ measurement at 1.5T using a PRESS-localized double quantum filter

• Published in: Magnetic resonance in medicine 2002-08, Vol.48 (2), p.233-241
• Main Authors: McLean, M.A., Busza, A.L., Wald, L.L., Simister, R.J., Barker, G.J., Williams, S.R.
• Format: Article
• Language: English
• Subjects: 1H-MRS; Adult; Biological and medical sciences; Brain Chemistry; Female; GABA; gamma-Aminobutyric Acid - analysis; human brain; Humans; Investigative techniques, diagnostic techniques (general aspects); Magnetic Resonance Spectroscopy - methods; Male; Medical sciences; multiple quantum filter; Nervous system; Radiodiagnosis. Nmr imagery. Nmr spectrometry; Reproducibility of Results; spectral editing
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.10208

A point‐resolved spectroscopy (PRESS)‐localized double quantum filter was implemented on a 1.5T clinical scanner for the estimation of γ‐amino butyric acid (GABA) concentrations in vivo. Several calibrations were found to be necessary for consistent results to be obtained. The apparent filter yield was approximately 38%; filter strength was sufficient to reduce the singlet metabolite peaks in vivo to below the level of the noise. Metabolite‐nulled experiments were performed, which confirmed that significant overlap occurred between macromolecule signals and the GABA resonance at 3.1 ppm. Although the multiplet arm at 2.9 ppm was confirmed to be relatively free of contamination with macromolecules, some contribution from these and from peptides is likely to remain; therefore, the term GABA+ is used. GABA+ concentrations were estimated relative to creatine (Cr) at the same echo time (TE) in a group of controls, studied on two occasions. The GABA+ concentration in 35‐ml regions of interest (ROIs) in the occipital lobe was found to be 1.4 ± 0.2 mM, with scan‐rescan repeatability of 38%. Magn Reson Med 48:233–241, 2002. © 2002 Wiley‐Liss, Inc.