Drosophila yolk protein produced in E. coli is accumulated by mosquito ovaries

Despite similar functions, the yolk proteins of the higher dipteran flies and the vitellogenins found in other insects are unrelated at the sequence level and have evolved from different genes. Both are selectively endocytosed into the ovary via receptors belonging to the LDLR receptor subfamily. We...

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Bibliographic Details
Published in:Insect molecular biology 2002-10, Vol.11 (5), p.487-496
Main Authors: Bownes, M., Hurd, H., Büsgen, T., Servay, D., Alvis, S., Popovic, B., Bruce, S., Burns, I., Rothwell, K., Walkinshaw, Malcolm
Format: Article
Language:English
Subjects:
Animals
Anopheles - metabolism
Cloning, Molecular
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
endocytosis
Escherichia coli
evolution
Female
Gene Expression
Genetic Vectors
Ovary - metabolism
reproduction
vitellogenesis
Vitellogenins - genetics
Vitellogenins - metabolism
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Summary:Despite similar functions, the yolk proteins of the higher dipteran flies and the vitellogenins found in other insects are unrelated at the sequence level and have evolved from different genes. Both are selectively endocytosed into the ovary via receptors belonging to the LDLR receptor subfamily. We cloned the Drosophila yp1 gene into an E. coli expression vector and showed that the yolk protein produced by E. coli is taken up into ovaries of both Drosophila melanogaster and the malaria mosquito Anopheles gambiae, which normally uses vitellogenin.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.2002.00357.x