A finite element model of cell deformation during magnetic bead twisting

1  Harvard School of Public Health, Boston, Massachusetts 02115; and 2  University of Kragujevac, 34000 Kragujevac, Yugoslavia Magnetic twisting cytometry probes mechanical properties of an adherent cell by applying a torque to a magnetic bead that is tightly bound to the cell surface. Here we have...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied physiology (1985) 2002-10, Vol.93 (4), p.1429-1436
Main Authors: Mijailovich, Srboljub M, Kojic, Milos, Zivkovic, Miroslav, Fabry, Ben, Fredberg, Jeffrey J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomechanics. Biorheology
Cell Adhesion - physiology
Cell Physiological Phenomena
Cell Size
Cells
Cells - cytology
Elasticity
Finite Element Analysis
Fundamental and applied biological sciences. Psychology
Magnetics
Magnetism
Microspheres
Models, Biological
Nonlinear Dynamics
Rotation
Space life sciences
Stress, Mechanical
Tissues, organs and organisms biophysics
Torque
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:1  Harvard School of Public Health, Boston, Massachusetts 02115; and 2  University of Kragujevac, 34000 Kragujevac, Yugoslavia Magnetic twisting cytometry probes mechanical properties of an adherent cell by applying a torque to a magnetic bead that is tightly bound to the cell surface. Here we have used a three-dimensional finite element model of cell deformation to compute the relationships between the applied torque and resulting bead rotation and lateral bead translation. From the analysis, we computed two coefficients that allow the cell elastic modulus to be estimated from measurements of either bead rotation or lateral bead translation, respectively, if the degree of bead embedding and the cell height are known. Although computed strains in proximity of the bead can be large, the relationships between applied torque and bead rotation or translation remain virtually linear up to bead rotations of 15°, above which geometrical nonlinearities become significant. This appreciable linear range stands in contrast to the intrinsically nonlinear force-displacement relationship that is observed when cells are indented during atomic force microscopy. Finally, these computations support the idea that adhesive forces are sufficient to keep the bead firmly attached to the cell surface throughout the range of working torques. elastic modulus; cell stiffness; bead embedding; cell height; magnetic twisting cytometry
ISSN:8750-7587
1522-1601
DOI:10.1152/japplphysiol.00255.2002