Genomic variations among Bartonella henselae isolates derived from naturally infected cats

• Published in: Veterinary microbiology 2002-10, Vol.89 (2), p.211-221
• Main Authors: Kabeya, Hidenori, Maruyama, Soichi, Irei, Mitsuhiro, Takahashi, Rena, Yamashita, Masaya, Mikami, Takeshi
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - blood; Bacteremia; Bacteremia - microbiology; Bacteremia - pathology; Bacteremia - veterinary; Bacteriology; Bartonella henselae; Bartonella henselae - genetics; Bartonella henselae - isolation & purification; Bartonella Infections - microbiology; Bartonella Infections - pathology; Bartonella Infections - veterinary; Biological and medical sciences; Carrier State - microbiology; Carrier State - pathology; Carrier State - veterinary; Cat Diseases - microbiology; Cat Diseases - pathology; Cat scratch disease; Cats; Colony Count, Microbial - veterinary; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field - veterinary; Epidemiology; Female; Fundamental and applied biological sciences. Psychology; Genetic diversity; Genetic Variation; Genetics; Longitudinal Studies; Male; Microbiology; PFGE; Polymerase Chain Reaction - veterinary; Polymorphism, Restriction Fragment Length; Relapsing; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/S0378-1135(02)00175-X

The purpose of this study was to understand the mechanisms of persistent infection with Bartonella henselae in cats. Blood samples were collected from three naturally infected cats for 24 months. These cats were confirmed to be persistently infected with B. henselae by serological and bacteriological examination. Relapsing bacteremia was found in all three cats with intervals of 3–19 months. Following the peaks of bacteremia, increases of specific antibody titer were observed in these cats. To examine the genetic differences among the isolates derived from the first and following bacteremia, the genome DNA patterns of the restriction enzyme fragment length polymorphism (RFLP) of the isolates were examined by pulsed field gel electrophoresis. The isolates derived from the first bacteremia showed an identical RFLP pattern in each of the three cats. The isolates derived from the following peaks, however, showed 1–3 of different RFLP patterns in these cats. Furthermore, the isolates showing different RFLP patterns from those of the first bacteremia were also detected at the following bacteremic peaks in all three cats examined. The 16S ribosomal RNA (rRNA) gene type of all isolates was found to be 16S rRNA type I. The emergence of genetically distinct organisms at various peaks of bacteremia may contribute to the establishment of persistent infection in the naturally infected cats.