Loading…
Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique
Fluorine-18 fluoro-2-deoxyglucose ((18)FDG) and carbon-14 2-deoxyglucose ((14)C-2-DG) are both widely used tracers of myocardial glucose uptake and phosphorylation. We have recently shown, using positron emission tomography (PET) and nuclear magnetic resonance, that ischaemia-reperfusion (I-R) cause...
Saved in:
| Published in: | European journal of nuclear medicine and molecular imaging 2002-10, Vol.29 (10), p.1334-1341 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c354t-dbdbb2b7957700dc66a08a137d270169d71beabfe151d35db09932571cc9a0aa3 |
|---|---|
| cites | |
| container_end_page | 1341 |
| container_issue | 10 |
| container_start_page | 1334 |
| container_title | European journal of nuclear medicine and molecular imaging |
| container_volume | 29 |
| creator | SOUTHWORTH, Richard DEARLING, Jason L. J MEDINA, Rodolfo A FLYNN, Aiden A PEDLEY, R. Barbara GARLICK, Pamela B |
| description | Fluorine-18 fluoro-2-deoxyglucose ((18)FDG) and carbon-14 2-deoxyglucose ((14)C-2-DG) are both widely used tracers of myocardial glucose uptake and phosphorylation. We have recently shown, using positron emission tomography (PET) and nuclear magnetic resonance, that ischaemia-reperfusion (I-R) causes differential changes in their uptake. We describe here the novel application of an autoradiographic technique allowing the investigation of this phenomenon at high resolution, using tracer concentrations of both analogues in the dual-perfused isolated rat heart. We also investigate the importance of glucose transporter (GLUT 1 and GLUT 4) distribution in governing the observed phosphorylated analogue accumulation. Hearts ( n=5) were perfused with Krebs buffer for 40 min, made regionally zero-flow ischaemic for 40 min and reperfused for 60 min with Krebs containing tracer (18)FDG (200 MBq) and tracer (14)C-2-DG (0.37 MBq). Hearts were then frozen and five sections (10 micro m) were cut per heart, fixed and exposed on phosphor storage plates for 18 h (for (18)FDG) and then for a further 9 days (for (14)C-2-DG). Quantitative digital images of tracer accumulation were obtained using a phosphor plate reader. The protocol was repeated in a second group of hearts and GLUT 1 and GLUT 4 distribution analysed. Post-ischaemic accumulation of (18)FDG-6-P was inhibited by 38.2%+/-1.7% and (14)C-DG-6-P by 19.0%+/-2.2%, compared with control ( P |
| doi_str_mv | 10.1007/s00259-002-0922-2 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72116418</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>818479791</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354t-dbdbb2b7957700dc66a08a137d270169d71beabfe151d35db09932571cc9a0aa3</originalsourceid><addsrcrecordid>eNpdkcuKFTEQhoMozkUfwI0Ewdm1ptKXdNwN4xUG3Og6VC59OmNOp03SyLyST2nGc5gRN5UK9f1_UfyEvAD2BhgTbzNjvJdNrQ2TnDf8ETmFAWQj2Cgf3_eCnZCznG8Yg5GP8ik5Ac4FdDCckt_vfc7ReCw-LjROdBc2E7OjJaFxiW5rwR-O4mL_nSx5janUsfW5JK-3v2q_0DI7mtyu_jCEW-qzmdHtvaldDFicpQkLnR2m8o7iugZv7jcjXdwviluJCa2Pu4TrXJXFmXnxPzf3jDyZMGT3_Piek-8fP3y7-txcf_305eryujFt35XGaqs110L2QjBmzTAgGxFaYblgMEgrQDvUk4MebNtbzaRseS_AGIkMsT0nFwffNcW6Nhe1r3e4EHBxcctKcIChg7GCr_4Db-KW6uVZceiGbuCsrRAcIJNizslNak1-j-lWAVN3KapDiqpWdZei4lXz8mi86b2zD4pjbBV4fQQwGwxTTcT4_MC11WccefsHj2-pPg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>214646203</pqid></control><display><type>article</type><title>Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique</title><source>Springer Nature</source><creator>SOUTHWORTH, Richard ; DEARLING, Jason L. J ; MEDINA, Rodolfo A ; FLYNN, Aiden A ; PEDLEY, R. Barbara ; GARLICK, Pamela B</creator><creatorcontrib>SOUTHWORTH, Richard ; DEARLING, Jason L. J ; MEDINA, Rodolfo A ; FLYNN, Aiden A ; PEDLEY, R. Barbara ; GARLICK, Pamela B</creatorcontrib><description>Fluorine-18 fluoro-2-deoxyglucose ((18)FDG) and carbon-14 2-deoxyglucose ((14)C-2-DG) are both widely used tracers of myocardial glucose uptake and phosphorylation. We have recently shown, using positron emission tomography (PET) and nuclear magnetic resonance, that ischaemia-reperfusion (I-R) causes differential changes in their uptake. We describe here the novel application of an autoradiographic technique allowing the investigation of this phenomenon at high resolution, using tracer concentrations of both analogues in the dual-perfused isolated rat heart. We also investigate the importance of glucose transporter (GLUT 1 and GLUT 4) distribution in governing the observed phosphorylated analogue accumulation. Hearts ( n=5) were perfused with Krebs buffer for 40 min, made regionally zero-flow ischaemic for 40 min and reperfused for 60 min with Krebs containing tracer (18)FDG (200 MBq) and tracer (14)C-2-DG (0.37 MBq). Hearts were then frozen and five sections (10 micro m) were cut per heart, fixed and exposed on phosphor storage plates for 18 h (for (18)FDG) and then for a further 9 days (for (14)C-2-DG). Quantitative digital images of tracer accumulation were obtained using a phosphor plate reader. The protocol was repeated in a second group of hearts and GLUT 1 and GLUT 4 distribution analysed. Post-ischaemic accumulation of (18)FDG-6-P was inhibited by 38.2%+/-1.7% and (14)C-DG-6-P by 19.0%+/-2.2%, compared with control ( P<0.05). After placing seven "lines of interrogation" across each heart section and analysing the phosphorylated tracer accumulation along them, a transmural gradient of both tracers was observed; this was highest at the endocardium and lowest at the epicardium. GLUT 4 translocated to the sarcolemma in the ischaemic/reperfused region (from 24%+/-3% to 59%+/-5%), while there was no cellular redistribution of GLUT 1. We conclude that since decreased phosphorylated tracer accumulation occurs after ischaemia-reperfusion, despite greater externalisation of GLUT 4, hexokinase or the affinities of the GLUT transporters are changed under these conditions.</description><identifier>ISSN: 1619-7070</identifier><identifier>EISSN: 1619-7089</identifier><identifier>DOI: 10.1007/s00259-002-0922-2</identifier><identifier>PMID: 12271416</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Autoradiography - methods ; Biological and medical sciences ; Carbon Radioisotopes - pharmacokinetics ; Cardiovascular system ; Cerebrovascular Circulation ; Deoxyglucose - pharmacokinetics ; Fluorodeoxyglucose F18 - pharmacokinetics ; Glucose - metabolism ; Glucose Transporter Type 1 ; Glucose Transporter Type 2 ; Heart Ventricles - metabolism ; Heart Ventricles - pathology ; In Vitro Techniques ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Monosaccharide Transport Proteins - metabolism ; Myocardial Ischemia - metabolism ; Myocardial Ischemia - pathology ; Myocardial Reperfusion ; Myocardium - metabolism ; Quality Control ; Radionuclide investigations ; Radiopharmaceuticals - pharmacokinetics ; Rats ; Rats, Wistar ; Reference Values ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>European journal of nuclear medicine and molecular imaging, 2002-10, Vol.29 (10), p.1334-1341</ispartof><rights>2003 INIST-CNRS</rights><rights>Springer-Verlag 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-dbdbb2b7957700dc66a08a137d270169d71beabfe151d35db09932571cc9a0aa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13922882$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12271416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SOUTHWORTH, Richard</creatorcontrib><creatorcontrib>DEARLING, Jason L. J</creatorcontrib><creatorcontrib>MEDINA, Rodolfo A</creatorcontrib><creatorcontrib>FLYNN, Aiden A</creatorcontrib><creatorcontrib>PEDLEY, R. Barbara</creatorcontrib><creatorcontrib>GARLICK, Pamela B</creatorcontrib><title>Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique</title><title>European journal of nuclear medicine and molecular imaging</title><addtitle>Eur J Nucl Med Mol Imaging</addtitle><description>Fluorine-18 fluoro-2-deoxyglucose ((18)FDG) and carbon-14 2-deoxyglucose ((14)C-2-DG) are both widely used tracers of myocardial glucose uptake and phosphorylation. We have recently shown, using positron emission tomography (PET) and nuclear magnetic resonance, that ischaemia-reperfusion (I-R) causes differential changes in their uptake. We describe here the novel application of an autoradiographic technique allowing the investigation of this phenomenon at high resolution, using tracer concentrations of both analogues in the dual-perfused isolated rat heart. We also investigate the importance of glucose transporter (GLUT 1 and GLUT 4) distribution in governing the observed phosphorylated analogue accumulation. Hearts ( n=5) were perfused with Krebs buffer for 40 min, made regionally zero-flow ischaemic for 40 min and reperfused for 60 min with Krebs containing tracer (18)FDG (200 MBq) and tracer (14)C-2-DG (0.37 MBq). Hearts were then frozen and five sections (10 micro m) were cut per heart, fixed and exposed on phosphor storage plates for 18 h (for (18)FDG) and then for a further 9 days (for (14)C-2-DG). Quantitative digital images of tracer accumulation were obtained using a phosphor plate reader. The protocol was repeated in a second group of hearts and GLUT 1 and GLUT 4 distribution analysed. Post-ischaemic accumulation of (18)FDG-6-P was inhibited by 38.2%+/-1.7% and (14)C-DG-6-P by 19.0%+/-2.2%, compared with control ( P<0.05). After placing seven "lines of interrogation" across each heart section and analysing the phosphorylated tracer accumulation along them, a transmural gradient of both tracers was observed; this was highest at the endocardium and lowest at the epicardium. GLUT 4 translocated to the sarcolemma in the ischaemic/reperfused region (from 24%+/-3% to 59%+/-5%), while there was no cellular redistribution of GLUT 1. We conclude that since decreased phosphorylated tracer accumulation occurs after ischaemia-reperfusion, despite greater externalisation of GLUT 4, hexokinase or the affinities of the GLUT transporters are changed under these conditions.</description><subject>Animals</subject><subject>Autoradiography - methods</subject><subject>Biological and medical sciences</subject><subject>Carbon Radioisotopes - pharmacokinetics</subject><subject>Cardiovascular system</subject><subject>Cerebrovascular Circulation</subject><subject>Deoxyglucose - pharmacokinetics</subject><subject>Fluorodeoxyglucose F18 - pharmacokinetics</subject><subject>Glucose - metabolism</subject><subject>Glucose Transporter Type 1</subject><subject>Glucose Transporter Type 2</subject><subject>Heart Ventricles - metabolism</subject><subject>Heart Ventricles - pathology</subject><subject>In Vitro Techniques</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Myocardial Ischemia - metabolism</subject><subject>Myocardial Ischemia - pathology</subject><subject>Myocardial Reperfusion</subject><subject>Myocardium - metabolism</subject><subject>Quality Control</subject><subject>Radionuclide investigations</subject><subject>Radiopharmaceuticals - pharmacokinetics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reference Values</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>1619-7070</issn><issn>1619-7089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNpdkcuKFTEQhoMozkUfwI0Ewdm1ptKXdNwN4xUG3Og6VC59OmNOp03SyLyST2nGc5gRN5UK9f1_UfyEvAD2BhgTbzNjvJdNrQ2TnDf8ETmFAWQj2Cgf3_eCnZCznG8Yg5GP8ik5Ac4FdDCckt_vfc7ReCw-LjROdBc2E7OjJaFxiW5rwR-O4mL_nSx5janUsfW5JK-3v2q_0DI7mtyu_jCEW-qzmdHtvaldDFicpQkLnR2m8o7iugZv7jcjXdwviluJCa2Pu4TrXJXFmXnxPzf3jDyZMGT3_Piek-8fP3y7-txcf_305eryujFt35XGaqs110L2QjBmzTAgGxFaYblgMEgrQDvUk4MebNtbzaRseS_AGIkMsT0nFwffNcW6Nhe1r3e4EHBxcctKcIChg7GCr_4Db-KW6uVZceiGbuCsrRAcIJNizslNak1-j-lWAVN3KapDiqpWdZei4lXz8mi86b2zD4pjbBV4fQQwGwxTTcT4_MC11WccefsHj2-pPg</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>SOUTHWORTH, Richard</creator><creator>DEARLING, Jason L. J</creator><creator>MEDINA, Rodolfo A</creator><creator>FLYNN, Aiden A</creator><creator>PEDLEY, R. Barbara</creator><creator>GARLICK, Pamela B</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique</title><author>SOUTHWORTH, Richard ; DEARLING, Jason L. J ; MEDINA, Rodolfo A ; FLYNN, Aiden A ; PEDLEY, R. Barbara ; GARLICK, Pamela B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-dbdbb2b7957700dc66a08a137d270169d71beabfe151d35db09932571cc9a0aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Autoradiography - methods</topic><topic>Biological and medical sciences</topic><topic>Carbon Radioisotopes - pharmacokinetics</topic><topic>Cardiovascular system</topic><topic>Cerebrovascular Circulation</topic><topic>Deoxyglucose - pharmacokinetics</topic><topic>Fluorodeoxyglucose F18 - pharmacokinetics</topic><topic>Glucose - metabolism</topic><topic>Glucose Transporter Type 1</topic><topic>Glucose Transporter Type 2</topic><topic>Heart Ventricles - metabolism</topic><topic>Heart Ventricles - pathology</topic><topic>In Vitro Techniques</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Myocardial Ischemia - metabolism</topic><topic>Myocardial Ischemia - pathology</topic><topic>Myocardial Reperfusion</topic><topic>Myocardium - metabolism</topic><topic>Quality Control</topic><topic>Radionuclide investigations</topic><topic>Radiopharmaceuticals - pharmacokinetics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reference Values</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SOUTHWORTH, Richard</creatorcontrib><creatorcontrib>DEARLING, Jason L. J</creatorcontrib><creatorcontrib>MEDINA, Rodolfo A</creatorcontrib><creatorcontrib>FLYNN, Aiden A</creatorcontrib><creatorcontrib>PEDLEY, R. Barbara</creatorcontrib><creatorcontrib>GARLICK, Pamela B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of nuclear medicine and molecular imaging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SOUTHWORTH, Richard</au><au>DEARLING, Jason L. J</au><au>MEDINA, Rodolfo A</au><au>FLYNN, Aiden A</au><au>PEDLEY, R. Barbara</au><au>GARLICK, Pamela B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique</atitle><jtitle>European journal of nuclear medicine and molecular imaging</jtitle><addtitle>Eur J Nucl Med Mol Imaging</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>29</volume><issue>10</issue><spage>1334</spage><epage>1341</epage><pages>1334-1341</pages><issn>1619-7070</issn><eissn>1619-7089</eissn><abstract>Fluorine-18 fluoro-2-deoxyglucose ((18)FDG) and carbon-14 2-deoxyglucose ((14)C-2-DG) are both widely used tracers of myocardial glucose uptake and phosphorylation. We have recently shown, using positron emission tomography (PET) and nuclear magnetic resonance, that ischaemia-reperfusion (I-R) causes differential changes in their uptake. We describe here the novel application of an autoradiographic technique allowing the investigation of this phenomenon at high resolution, using tracer concentrations of both analogues in the dual-perfused isolated rat heart. We also investigate the importance of glucose transporter (GLUT 1 and GLUT 4) distribution in governing the observed phosphorylated analogue accumulation. Hearts ( n=5) were perfused with Krebs buffer for 40 min, made regionally zero-flow ischaemic for 40 min and reperfused for 60 min with Krebs containing tracer (18)FDG (200 MBq) and tracer (14)C-2-DG (0.37 MBq). Hearts were then frozen and five sections (10 micro m) were cut per heart, fixed and exposed on phosphor storage plates for 18 h (for (18)FDG) and then for a further 9 days (for (14)C-2-DG). Quantitative digital images of tracer accumulation were obtained using a phosphor plate reader. The protocol was repeated in a second group of hearts and GLUT 1 and GLUT 4 distribution analysed. Post-ischaemic accumulation of (18)FDG-6-P was inhibited by 38.2%+/-1.7% and (14)C-DG-6-P by 19.0%+/-2.2%, compared with control ( P<0.05). After placing seven "lines of interrogation" across each heart section and analysing the phosphorylated tracer accumulation along them, a transmural gradient of both tracers was observed; this was highest at the endocardium and lowest at the epicardium. GLUT 4 translocated to the sarcolemma in the ischaemic/reperfused region (from 24%+/-3% to 59%+/-5%), while there was no cellular redistribution of GLUT 1. We conclude that since decreased phosphorylated tracer accumulation occurs after ischaemia-reperfusion, despite greater externalisation of GLUT 4, hexokinase or the affinities of the GLUT transporters are changed under these conditions.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12271416</pmid><doi>10.1007/s00259-002-0922-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1619-7070 |
| ispartof | European journal of nuclear medicine and molecular imaging, 2002-10, Vol.29 (10), p.1334-1341 |
| issn | 1619-7070 1619-7089 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72116418 |
| source | Springer Nature |
| subjects | Animals Autoradiography - methods Biological and medical sciences Carbon Radioisotopes - pharmacokinetics Cardiovascular system Cerebrovascular Circulation Deoxyglucose - pharmacokinetics Fluorodeoxyglucose F18 - pharmacokinetics Glucose - metabolism Glucose Transporter Type 1 Glucose Transporter Type 2 Heart Ventricles - metabolism Heart Ventricles - pathology In Vitro Techniques Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Monosaccharide Transport Proteins - metabolism Myocardial Ischemia - metabolism Myocardial Ischemia - pathology Myocardial Reperfusion Myocardium - metabolism Quality Control Radionuclide investigations Radiopharmaceuticals - pharmacokinetics Rats Rats, Wistar Reference Values Reproducibility of Results Sensitivity and Specificity |
| title | Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T13%3A59%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dissociation%20of%20glucose%20tracer%20uptake%20and%20glucose%20transporter%20distribution%20in%20the%20regionally%20ischaemic%20isolated%20rat%20heart:%20application%20of%20a%20new%20autoradiographic%20technique&rft.jtitle=European%20journal%20of%20nuclear%20medicine%20and%20molecular%20imaging&rft.au=SOUTHWORTH,%20Richard&rft.date=2002-10-01&rft.volume=29&rft.issue=10&rft.spage=1334&rft.epage=1341&rft.pages=1334-1341&rft.issn=1619-7070&rft.eissn=1619-7089&rft_id=info:doi/10.1007/s00259-002-0922-2&rft_dat=%3Cproquest_cross%3E818479791%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c354t-dbdbb2b7957700dc66a08a137d270169d71beabfe151d35db09932571cc9a0aa3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=214646203&rft_id=info:pmid/12271416&rfr_iscdi=true |