Melatonin protects against oxidized low-density lipoprotein-induced inhibition of nitric oxide production in human umbilical artery

We evaluated the antioxidative effect of melatonin on the oxidized low‐density lipoprotein (LDL)‐induced impairment of nitric oxide (NO) production in human umbilical artery, which may be the prime cause of endothelial dysfunction in pre‐eclampsia. Umbilical artery sections with intact endothelium w...

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Published in:Journal of pineal research 2001-10, Vol.31 (3), p.281-288
Main Authors: Wakatsuki, Akihiko, Okatani, Yuji, Ikenoue, Nobuo, Shinohara, Koichi, Watanabe, Kazushi, Fukaya, Takao
Format: Article
Language:English
Subjects:
Adult
Antioxidants - pharmacology
Arginine - pharmacology
Biological and medical sciences
Chromatography, High Pressure Liquid
Diseases of mother, fetus and pregnancy
Enzyme-Linked Immunosorbent Assay
Female
Free Radical Scavengers - pharmacology
Gynecology. Andrology. Obstetrics
Humans
Lipoproteins, LDL - pharmacology
Male
Medical sciences
melatonin
Melatonin - pharmacology
nitric oxide
Nitric Oxide - biosynthesis
Oxidation-Reduction
oxidized LDL
Pregnancy
Pregnancy. Fetus. Placenta
Umbilical Arteries - drug effects
Umbilical Arteries - metabolism
umbilical artery
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Summary:We evaluated the antioxidative effect of melatonin on the oxidized low‐density lipoprotein (LDL)‐induced impairment of nitric oxide (NO) production in human umbilical artery, which may be the prime cause of endothelial dysfunction in pre‐eclampsia. Umbilical artery sections with intact endothelium were obtained from healthy pregnant women who were delivered between 37 and 40 wk of gestation. The production of NO in the umbilical arteries was stimulated by adding l‐arginine followed by incubation for 60 min. NO concentrations were estimated by measuring nitrite ions (NO2−) using high‐performance liquid chromatography. LDL was oxidized by incubation with 5 μM CuSO4 at 37°C for 4 hr, followed by dialysis at 4°C for 24 hr. Prior to the addition of l‐arginine, the segments were treated with native or oxidized LDL (0, 50, 100, 200, 400 μg/mL), or were pre‐treated with either mannitol (50 mM) or melatonin (20, 100, 500 μM) before adding oxidized LDL. Changes in l‐arginine‐induced NO2− production were expressed as a percentage of NO2− production at the end of pre‐incubation. Treatment with oxidized LDL significantly reduced l‐arginine‐induced NO2− production (P
ISSN:0742-3098
1600-079X
DOI:10.1034/j.1600-079X.2001.310313.x