Nisin production from Lactococcus lactis A.T.C.C. 7962 using supplemented whey permeate

• Published in: Biotechnology and applied biochemistry 2001-10, Vol.34 (2), p.103-107
• Main Authors: FLORES, Simone Hickmann, ALEGRE, Ranulfo Monte
• Format: Article
• Language: English
• Subjects: Animals; Antibiotics; Biological and medical sciences; Biomass; Biotechnology; Cattle; Cheese; Culture Media; Escherichia coli Proteins; Fermentation; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; Hydrogen-Ion Concentration; Industrial applications and implications. Economical aspects; Lactococcus lactis; Lactococcus lactis - growth & development; Lactococcus lactis - metabolism; Membrane Transport Proteins - metabolism; Milk Proteins - metabolism; Monosaccharide Transport Proteins; Nisin - biosynthesis; Nisin - metabolism; Production of active biomolecules; Symporters; Whey Proteins
• ISSN: 0885-4513; 1470-8744
• DOI: 10.1042/BA20010030

The influence of pH control and aeration (20% dissolved oxygen) on nisin production in a supplemented cheese whey permeate was examined during batch fermentation with Lactococcus lactis subsp. lactis A.T.C.C. 7962. A maximum nisin activity of 5280 i.u./ml of medium was observed in the raw extract of nisin after 9 h of fermentation with a constant pH at 4.9. However, the fermentation was continued until 24 h, when a decrease in the nisin activity was observed. The pH control did not influence the nisin production and aeration of the culture medium increased cell growth (biomass) but not nisin activity. The yeast Kluyveromyces marxianus, used as an alternative method to control pH, has not been efficient.