Fcgamma receptor-mediated phagocytosis of Plasmodium falciparum-infected erythrocytes in vitro

Although convincing evidence exists for the role of immunoglobulin G (IgG) antibodies in immunity to malaria, antibody titres do not usually predict protection. In this study we have assessed the interaction between Plasmodium falciparum-infected erythrocytes (PE), opsonized with immune serum contai...

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Bibliographic Details
Published in:Clinical and experimental immunology 2002-11, Vol.130 (2), p.300-306
Main Authors: Tebo, A E, Kremsner, P G, Luty, A J F
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
Antibodies, Protozoan - immunology
Cell Line
Child
Erythrocytes - parasitology
Humans
Immunoglobulin G - blood
Immunoglobulin G - immunology
Malaria, Falciparum - immunology
Middle Aged
Monocytes - immunology
Opsonin Proteins - immunology
Phagocytosis
Plasmodium falciparum
Receptors, IgG - physiology
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Summary:Although convincing evidence exists for the role of immunoglobulin G (IgG) antibodies in immunity to malaria, antibody titres do not usually predict protection. In this study we have assessed the interaction between Plasmodium falciparum-infected erythrocytes (PE), opsonized with immune serum containing different amounts of IgG antibody isotypes, with either THP-1 cells, ex-vivo human monocytes or IIAI.6 transfectant cells expressing Fc(gamma)RIIa-Arg/Arg131 or -His/His131 allotypes. Our results show that PMA-treated THP-1 cells were capable of phagocytosing serum-opsonized PE by Fc(gamma)RI (CD64) and Fc(gamma)RIIa (CD32), acting synergistically. The known Fc(gamma)RIIa polymorphism motivated us to examine its influence on IgG isotype-mediated phagocytosis of opsonized PE with human monocytes and the IIAI.6 transfectant cells expressing either allelic forms. Regardless of the cell type, PE phagocytosis with Fc(gamma)RIIa-His/His131 was highest following opsonization with a predominantly IgG3-containing immune serum pool. In contrast, PE phagocytosis with Fc(gamma)RIIa-Arg/Arg131 tended to be higher with an IgG1-containing pool. These results suggest a genetically determined influence of effector cell phenotype on IgG antibody-pathogen interaction in P. falciparum malaria.
ISSN:0009-9104