Trafficking of Human ADAM 12-L: Retention in the trans-Golgi Network

We have investigated the trafficking of the membrane-anchored form of human ADAM 12 (ADAM 12-L) fused to a green fluorescence protein tag. Subcellular localization of the protein in transiently transfected cells was determined by fluorescence microscopy and trypsin sensitivity. Full-length ADAM 12-L...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-08, Vol.275 (2), p.261-267
Main Authors: Hougaard, Susanne, Loechel, Frosty, Xu, Xiufeng, Tajima, Rie, Albrechtsen, Reidar, Wewer, Ulla M.
Format: Article
Language:English
Subjects:
ADAM 12
ADAM Proteins
ADAM12 Protein
Animals
Biological Transport
Cell Compartmentation
Cell Membrane - metabolism
CHO Cells
COS Cells
Cricetinae
Cytoplasm - metabolism
disintegrin
Golgi Apparatus - metabolism
HeLa Cells
Humans
Immunohistochemistry
Membrane Proteins - biosynthesis
Membrane Proteins - metabolism
Metalloendopeptidases - biosynthesis
Metalloendopeptidases - metabolism
metalloprotease
Microscopy, Fluorescence
Octoxynol
trafficking
trans-Golgi network
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have investigated the trafficking of the membrane-anchored form of human ADAM 12 (ADAM 12-L) fused to a green fluorescence protein tag. Subcellular localization of the protein in transiently transfected cells was determined by fluorescence microscopy and trypsin sensitivity. Full-length ADAM 12-L was retained in a perinuclear compartment, which was shown to be the trans-Golgi network. In contrast, ADAM 12-L lacking the cytoplasmic domain reached the cell surface. Based on analysis of deletions and mutations of the cytoplasmic tail of ADAM 12-L, the retention signal is comprised of both the cytoplasmic and transmembrane domains, but not the Src homology 3 domain (SH3) binding sites. These results raise the possibility that a trafficking checkpoint in the trans-Golgi network is one of the cellular mechanisms for regulation of ADAM 12-L function, by allowing a rapid release of ADAM 12-L to the cell surface under specific stimuli.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.3295