Mutations of the bak gene in human gastric and colorectal cancers

The Bcl-2 homologue Bak is a potent inducer of apoptosis. We performed PCR-based single-strand conformational polymorphism and sequencing analysis of the entire coding region of the bak gene (exons 2-6) in 24 primary gastric cancers (6 early-stage and 18 advanced-stage cancers) and 20 primary colore...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2000-08, Vol.60 (16), p.4328-4330
Main Authors: KONDO, S, SHINOMURA, Y, MIYAZAKI, Y, KIYOHARA, T, TSUTSUI, S, KITAMURA, S, NAGASAWA, Y, NAKAHARA, M, KANAYAMA, S, MATSUZAWA, Y
Format: Article
Language:English
Subjects:
bak gene
bcl-2 Homologous Antagonist-Killer Protein
Biological and medical sciences
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
DNA Mutational Analysis
Exons - genetics
gastric carcinoma
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Medical sciences
Membrane Proteins - genetics
Mutation, Missense
Neoplasm Staging
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:The Bcl-2 homologue Bak is a potent inducer of apoptosis. We performed PCR-based single-strand conformational polymorphism and sequencing analysis of the entire coding region of the bak gene (exons 2-6) in 24 primary gastric cancers (6 early-stage and 18 advanced-stage cancers) and 20 primary colorectal cancers (6 early-stage and 14 advanced-stage cancers). The data herein demonstrate, for the first time, the mutation of the bak gene in gastric and colorectal cancers. Missense bak gene mutations were observed in 3 of 24 (12.5%) gastric cancers and 2 of 20 (10.0%) colorectal cancers. Sequence alterations without amino acid alteration were observed 1 of 24 (4.2%) gastric cancers and 2 of 20 (10.0%) colorectal cancers. Mutations in the bak gene were observed only in advanced-stage gastrointestinal cancers but not in early-stage cancers. Our observations suggest that mutations in this gene predispose bearers to the development of gastrointestinal malignancies in at least a subset of the cases.
ISSN:0008-5472
1538-7445