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Isolation and identification of the group B streptococcal toxin CM101 from infants with sepsis

Objective: To determine whether the group B streptococcal (GBS) polysaccharide exotoxin CM101, which induces a complement-activated cytokine-driven inflammatory response, is present in body fluids of infants with GBS disease. Study design: With a sandwich enzyme-linked immunosorbent assay, CM101 was...

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Published in:The Journal of pediatrics 2000-09, Vol.137 (3), p.338-344
Main Authors: Sundell, Håkan W., Yan, Heping, Carter, Clint E., Wamil, Barbara D., Wu, Kanning, Gaddipati, Rao, Li, Dongbei, Hellerqvist, Carl G.
Format: Article
Language:English
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Summary:Objective: To determine whether the group B streptococcal (GBS) polysaccharide exotoxin CM101, which induces a complement-activated cytokine-driven inflammatory response, is present in body fluids of infants with GBS disease. Study design: With a sandwich enzyme-linked immunosorbent assay, CM101 was measured in plasma, urine, and cerebrospinal fluid from newborn infants who were evaluated for possible infection and from older infants with culture-confirmed GBS disease. Results: Urine from 11 newborn infants with culture-confirmed early-onset disease contained large amounts of CM101 (1.0 to 5.5 mg/48 h). Plasma concentrations were 62.6 ± 10.5 μg/mL in these infants and were 69.0 ± 21.2 μg/mL in 4 older infants with late-onset disease. Plasma CM101 concentrations did not correlate with indexes of illness severity, leukocyte counts, or interleukin-6 or interleukin-8 plasma concentrations. CM101 was present in cerebrospinal fluid of 5 infants with meningitis (8.4 ± 1.6 μg/mL). CM101 was not found in control samples. CM101 isolated from urine had molecular weight and sugar composition similar to those obtained from GBS culture media, and they both elicited a comparable pathophysiologic response when infused intravenously in lambs. Conclusions: CM101 is present in infants with GBS disease, and it appears to be the same as CM101 obtained from GBS culture media. (J Pediatr 2000;137:338-44)
ISSN:0022-3476
1097-6833
DOI:10.1067/mpd.2000.107839