The personal identification of many samples recovered from under the sea

An automatic and rapid DNA typing system was employed for personal identification, using fragmentary tissue samples from victims in an airplane accident. Two victims were crushed into small pieces, and 33 samples suspected to belong to them were recovered from under the sea. From each sample, 10 mg...

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Bibliographic Details
Published in:Forensic science international 2000-09, Vol.113 (1), p.79-85
Main Authors: Mukaida, Masahiro, Kimura, Hiroko, Takada, Yuzo, Masuda, Tomoo, Nakata, Yasuko
Format: Article
Language:English
Subjects:
ABO Blood-Group System - genetics
ABO system
Accidents, Aviation
Biological and medical sciences
Blood groups
Blood Stains
Classical genetics, quantitative genetics, hybrids
DNA Fingerprinting - methods
DNA, Mitochondrial - genetics
Enzymes
Female
Fragmentary tissue
Fundamental and applied biological sciences. Psychology
Gene polymorphism
Genetics of eukaryotes. Biological and molecular evolution
Genotype
Hair - cytology
Human
Human tissues
Humans
Identification
Male
Marine animals
Marine organisms
Minisatellite Repeats - genetics
Mitochondrial DNA
Paternity
PCR–SSP
Polymorphism
Polymorphism, Genetic - genetics
Seawater
Stains & staining
STR-marker
TaqMan PCR
Time Factors
Tissues
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Summary:An automatic and rapid DNA typing system was employed for personal identification, using fragmentary tissue samples from victims in an airplane accident. Two victims were crushed into small pieces, and 33 samples suspected to belong to them were recovered from under the sea. From each sample, 10 mg was used for testing. The parents’ bloods of two presumptive victims were also examined. DNA extraction from samples was performed by the NaI method, and the obtained DNA samples were analyzed with the ABI PRISM system. Among 33 samples, 31 samples were identified to be human tissues, possibly from two victims. The other two samples seemed to be parts of marine animals. ABO blood group, STR polymorphism, and mitochondrial DNA polymorphism typing were possible in every examined human sample. Two victims’ fragmentary tissues were identified by determining ABO genotype, STR type and mitochondrial DNA type. The system we employed enabled an accurate typing of many fragmentary samples in a short time, thus contributing to the fast and secure identification of many victims in such cases as big air accidents.
ISSN:0379-0738
1872-6283
DOI:10.1016/S0379-0738(00)00219-X