Dynamic regulation of prothoracic gland ecdysteroidogenesis: Manduca sexta recombinant prothoracicotropic hormone and brain extracts have identical effects

Multiple assays were conducted in order to determine if the recently available recombinant prothoracicotropic hormone (rPTTH) from Manduca sexta is identical, or similar, to the natural hormone and if results from its use in a variety of assays confirm, or are inconsistent with, previous studies ove...

Full description

Saved in:
Bibliographic Details
Published in:Insect biochemistry and molecular biology 2000-11, Vol.30 (11), p.1079-1089
Main Authors: Gilbert, L.I, Rybczynski, R, Song, Q, Mizoguchi, A, Morreale, R, Smith, W.A, Matubayashi, H, Shionoya, M, Nagata, S, Kataoka, H
Format: Article
Language:English
Subjects:
Animals
Brain - physiology
Brain hormone
Cyclic AMP - metabolism
Cyclic AMP, stimulation of
Ecdysteroidogenesis, control of
Ecdysteroids
Immunohistochemistry
Insect Hormones - pharmacology
Manduca - physiology
Manduca sexta
Molting - physiology
Molting, control of
Recombinant Proteins - pharmacology
S6 phosphorylation
Steroids - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Multiple assays were conducted in order to determine if the recently available recombinant prothoracicotropic hormone (rPTTH) from Manduca sexta is identical, or similar, to the natural hormone and if results from its use in a variety of assays confirm, or are inconsistent with, previous studies over the past 20 years on PTTH action using brain extract. Brain extracts and rPTTH showed similar, if not identical, effects on the cell biology of Manduca prothoracic gland cells with the following results: increased levels of cAMP (adenosine 3′:5′ cyclic monophosphate) synthesis; requirement for extracellular Ca 2+ in in vitro studies; ecdysteroidogenesis stimulation in vitro; stimulation of general and specific protein synthesis; immunocytochemical identification of the two lateral cells in each brain hemisphere as the source of PTTH (the prothoracicotropes); the ability of antibodies to rPTTH to inhibit ecdysteroidogenesis stimulation in vitro; and the multiple phosphorylation of the ribosomal protein S6. The data revealed that brain extract and rPTTH show equivalent effects in all of the assays, indicating that this rPTTH is the natural PTTH of Manduca and that the data generated with brain extracts over the past two decades are indeed relevant.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(00)00083-7