Enhanced Levels of the Aroma and Flavor Compound S-Linalool by Metabolic Engineering of the Terpenoid Pathway in Tomato Fruits

The aromas of fruits, vegetables, and flowers are mixtures of volatile metabolites, often present in parts per billion levels or less. We show here that tomato (Lycopersicon esculentum Mill.) plants transgenic for a heterologous Clarkia breweri S-linalool synthase (LIS) gene, under the control of th...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 2001-11, Vol.127 (3), p.1256-1265
Main Authors: Lewinsohn, Efraim, Fernond Schalechet, Wilkinson, Jack, Kenji Matsui, Tadmor, Yaakov, Kyoung-Hee Nam, Orit Amar, Elena Lastochkin, Olga Larkov, Uzi Ravid, Hiatt, William, Gepstein, Shimon, Pichersky, Eran
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Alcohols
beta Carotene - metabolism
Biochemical Processes and Macromolecular Structures
Biological and medical sciences
Carotenoids - metabolism
Diphosphates
Flavors
Food Technology
Fruit - chemistry
Fruit - genetics
Fruit - metabolism
Fruits
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Genetic Engineering
Genetics and breeding of economic plants
Hydro-Lyases - genetics
Hydro-Lyases - metabolism
linalool
Lutein - metabolism
Lycopersicon esculentum
Lycopersicon esculentum - chemistry
Lycopersicon esculentum - genetics
Lycopersicon esculentum - metabolism
Metabolites
Monoterpenes
Oils, Volatile
Phenotype
Plants
Plants, Genetically Modified
Ripening
Terpenes - chemistry
Terpenes - metabolism
Terpenoids
Tocopherols - metabolism
Tomatoes
Transgenic plants
Varietal selection. Specialized plant breeding, plant breeding aims
Yield, quality, earliness, varia
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aromas of fruits, vegetables, and flowers are mixtures of volatile metabolites, often present in parts per billion levels or less. We show here that tomato (Lycopersicon esculentum Mill.) plants transgenic for a heterologous Clarkia breweri S-linalool synthase (LIS) gene, under the control of the tomato late-ripening-specific E8 promoter, synthesize and accumulate S-linalool and 8-hydroxylinalool in ripening fruits. Apart from the difference in volatiles, no other phenotypic alterations were noted, including the levels of other terpenoids such as γ- and α-tocopherols, lycopene, β-carotene, and lutein. Our studies indicate that it is possible to enhance the levels of monoterpenes in ripening fruits by metabolic engineering.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.010293