Alu repeat sequences are present in increased proportions compared to a unique gene in plasma/serum DNA: evidence for a preferential release from viable cells?

Small amounts of DNA circulate freely in plasma or serum, but the mechanism of release is not known. To determine if DNA is actively excreted from viable cells, we utilized real-time PCR to measure the proportion of Alu repeat sequences compared to the beta-globin gene in serum and lymphocyte DNA in...

Full description

Saved in:
Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2001-09, Vol.945 (1), p.258-264
Main Authors: Stroun, M, Lyautey, J, Lederrey, C, Mulcahy, H E, Anker, P
Format: Article
Language:English
Subjects:
Base Sequence
DNA - blood
DNA - genetics
DNA Primers
Globins - genetics
Humans
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Small amounts of DNA circulate freely in plasma or serum, but the mechanism of release is not known. To determine if DNA is actively excreted from viable cells, we utilized real-time PCR to measure the proportion of Alu repeat sequences compared to the beta-globin gene in serum and lymphocyte DNA in 27 cancer patients and 22 healthy controls. The proportion of Alu compared to beta-globin was significantly greater in serum DNA than in lymphocyte DNA both in control subjects (p = 0.003) and in cancer patients (p < 0.001). Overall, the proportion was similar in cancer and control patients (p = 0.79). Further experiments showed that the beta-globin gene was not more vulnerable to degradation by nuclease action than Alu sequences. Our results lead us to conclude that active DNA release is likely to play a significant role in the origin of circulating DNA.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.2001.tb03894.x