A quantitative analysis of cortical spreading depression events in the feline brain characterized with diffusion-weighted MRI

• Published in: Journal of magnetic resonance imaging 2000-11, Vol.12 (5), p.722-733
• Main Authors: Bockhorst, K.H.J., Smith, J.M., Smith, M.I., Bradley, D.P., Houston, G.C., Carpenter, T.A, Hall, L.D., Papadakis, N.G., Parsons, A.A., Huang, C.L.-H., James, M.F.
• Format: Article
• Language: English
• Subjects: Animals; Brain Mapping - methods; Cats; Cerebral Cortex - physiology; Cortical Spreading Depression; diffusion-weighted MRI; Echo-Planar Imaging - methods; Female; migraine; Models, Animal; Probability; Sensitivity and Specificity; stroke
• ISSN: 1053-1807; 1522-2586
• DOI: 10.1002/1522-2586(200011)12:5<722::AID-JMRI9>3.0.CO;2-A

Cortical spreading depression (CSD) in the gyrencephalic cat brain was detected with diffusion‐weighted echoplanar (DWEP) magnetic resonance imaging (4–8/min for 1–2 hours) using a horizontal imaging plane through the suprasylvian (SG) and marginal gyri. A t‐statistic mapping technique allowed a quantitative characterization of the passage of events through single‐image pixels (0.15 mm2), thus providing a resolution unavailable to previous studies in which time‐dependent changes instead were derived from averaging data over relatively large ROIs. Using the enhanced analysis, CSD events initiated by KCl could be quantified for the first time as primary or secondary according to their spatial and temporal features. Primary events covered 26.2 ± 9.9 mm2of cortical surface (mean ± SD, n = 7 experiments) and propagated rapidly (3.5 ± 0.65 mm • min−1) with a hemispherical geometry. In contrast, the subsequent secondary events were multiple, spatially restricted (covering 7.6 ± 4.6 mm2, P < 0.005), slower in propagation (2.6 ± 0.41 mm • min−1, P < 0.012), and often confined to the originating gyrus (26 out of 59 events). However, both event types were associated with significantly reduced apparent diffusion coefficients (ADCs; from 800 to ∼660 × 10−6 mm2• s−1, P < 0.05) that were similar for both primary (21 ± 5.1%) and secondary waves (18 ± 7.7%) and that had similar durations (full width at half‐maximal height: 86 ± 17 vs. 79 ± 20 seconds, respectively). These findings associate CSD for the first time with two categories of ADC disturbance that are similar in amplitude and duration but that differ in spatial extent, velocity, and extensiveness of spread. J. Magn. Reson. Imaging 2000;12:722–733. © 2000 Wiley‐Liss, Inc.