Multielement electrothermal atomic absorption spectrometry: A study on direct and simultaneous determination of chromium and manganese in urine

A study was carried out on the direct determination of Cr and Mn in urine using simultaneous atomic absorption spectrometry (SIMAAS). The heating program conditions, the absorbance signal profiles, the influence of different chemical modifiers, and the urine sample volume delivery into the tube were...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2001-12, Vol.371 (7), p.909-914
Main Authors: OLIVEIRA, P. V, OLIVEIRA, E
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Atomic absorption spectroscopy
Biological and medical sciences
Calibration
Chemical properties
Chromium - urine
Female
Fundamental and applied biological sciences. Psychology
Humans
Inorganic compounds
Male
Manganese - urine
Other biological molecules
Sensitivity and Specificity
Smoking - urine
Spectrophotometry, Atomic - methods
Spectrophotometry, Atomic - standards
Trace Elements - urine
Urine
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Summary:A study was carried out on the direct determination of Cr and Mn in urine using simultaneous atomic absorption spectrometry (SIMAAS). The heating program conditions, the absorbance signal profiles, the influence of different chemical modifiers, and the urine sample volume delivery into the tube were optimized to perform the calibration with aqueous solutions. Among several chemical modifiers tested, the best recovery and repeatability results were obtained for 3 microg Mg(NO3)2. On using this modifier, the pyrolysis and atomization temperatures for simultaneous determination of Cr and Mn were 1,300 degrees C and 2,500 degrees C, respectively. Urine samples were diluted (1+1) with 2.0% (v/v) HNO3 + 0.05% (w/v) Triton X-100 prepared in high purity water. A 20-microL aliquot of analytical solution and 10 microL of chemical modifier solution were delivered to the graphite tube. The characteristic masses were 7.8 pg for Cr (RSD=4.0%) and 4.6 pg for Mn (RSD=2.6%). The limits of detection were 0.08 microg L(-1) (n=20, 3s) for Cr and 0.16 microg L(-1) (n=20, 3s) for Mn. Recovery studies for 1.0 or 2.5 microg L(-1) of Cr and Mn added to different urine samples showed acceptable results for Cr (100%, RSD=14%) and Mn (88%, RSD=5.6%).
ISSN:0937-0633
1618-2642
1432-1130
DOI:10.1007/s002160101060