The role of the antennal glands in ion and body volume regulation of cannulated Penaeus monodon reared in various salinity conditions

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2000-10, Vol.127 (2), p.121-129
• Main Authors: Lin, Shian-Chuann, Liou, Chyng-Hwa, Cheng, Jin-Hua
• Format: Article
• Language: English
• Subjects: Animals; Body volume; Cannulation; Hemolymph; Hemolymph - chemistry; Ions; Marine; Osmolar Concentration; Osmoregulation; Penaeidae - anatomy & histology; Penaeus monodon; Salinity; Seawater; Shrimp; Sodium Chloride; Urine; Urine - chemistry
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/S1095-6433(00)00245-2

Urinary production rate and the osmotic and ionic concentrations in both urine and hemolymph were measured in cannulated intermolt Penaeus monodon which were either abruptly transferred from 45 ppt seawater to 15 ppt seawater (Experiment 1) or acclimated to 5, 25 and 45 ppt seawater (Experiment 2). In Experiment 1, urinary magnesium concentration fell dramatically from 228 to 30 mEq/l within 4 h post-transfer, but 8 h after transfer, U/ H (urine/hemolymph) ratios stabilized at between 1.0 and 2.5. Sodium was higher in urine than in hemolymph during the first 24 h after transfer, while potassium was lower in urine than in hemolymph until 72 h after transfer, which suggests that sodium and potassium concentrations are regulated by the antennal gland after an abrupt change in media. In Experiment 2, the urinary production rate of P. monodon decreased as salinity increased, suggesting that the antennal glands also regulate body volume. In the acclimated shrimps of Experiment 2, the antennal glands did not appear to regulate osmolarity or the concentration of chloride, sodium, potassium, and calcium ions, but as salinity increased, U/ H ratios of magnesium increased from 2.3 to13.5, and active secretion by the antennal gland accounted for 57∼93% of the total magnesium excretion through urine. These results suggest that active secretion of magnesium by the antennal gland enable this shrimp to maintain hypoionic levels of magnesium in the hemolymph.