Carotenoid dependence of the protochlorophyllide to chlorophyllide phototransformation in dark-grown wheat seedlings

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2001-12, Vol.65 (2), p.171-176
• Main Authors: Yahubyan, Galina, Minkov, Ivan, Sundqvist, Christer
• Format: Article
• Language: English
• Subjects: Carotenoids; Carotenoids - metabolism; Chlorophyllide; Chlorophyllides - metabolism; Darkness; Light; Norflurazon; Oxidation-Reduction; Partial photoreduction; Protochlorophyllide; Protochlorophyllide - metabolism; Pyridazines - pharmacology; Seeds - drug effects; Seeds - growth & development; Spectral forms; Spectrometry, Fluorescence; Triticum - drug effects; Triticum - growth & development; Triticum - metabolism
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/S1011-1344(01)00266-4

The influence of carotenoids on partial protochlorophyllide (Pchlide) photoreduction and the successive formation of long-wavelength chlorophyllide (Chlide) forms was studied by low-temperature fluorescence spectroscopy (77 K). Wheat leaves with a decreased content of carotenoids obtained from norflurazon-treated seedlings (10 and 100 μmol l −1) were compared with leaves containing normal amounts of these pigments. Partial photoreduction of Pchlide was achieved by irradiation of the leaves with one light flash in combination with a number of neutral gray and/or red Perspex filters. There were significant differences between the fluorescence emission spectra (the position and height of the peaks) of dark-grown normal and carotenoid-deficient leaves irradiated with non-saturating white light of increasing intensity. The long-wavelength Chlide forms appeared first in the leaves nearly devoid of carotenoids (treated with 100 μmol l −1 norflurazon), then in the leaves with carotenoid deficiency (treated with 10 μmol l −1 norflurazon), and finally in normal leaves. After irradiation with non-saturating light of the same intensity, the ratio Chlide/Pchlide 657 was always the highest in the leaves nearly deficient of carotenoids, medium in the leaves with carotenoid deficiency and lowest in the normal leaves. Similarly to white light, red light of low intensity induced faster formation of long-wavelength Chlide species in the leaves with carotenoid deficiency in comparison to the normal leaves. We propose that, in leaves with reduced carotenoid content, a greater number of Pchlide molecules transform to Chlide per light flash than in normal leaves. The results are discussed in relation to the involvement of carotenoids in competitive absorption and light screening, as well as to their influence on Pchlide–Chlide interactions.