Shape instability of a biomembrane driven by a local softening of the underlying actin cortex

• Published in: Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics Statistical physics, plasmas, fluids, and related interdisciplinary topics, 2000-09, Vol.62 (3 Pt B), p.3974-3985
• Main Authors: Boulbitch, A, Simson, R, Simson, D A, Merkel, R, Häckl, W, Bärmann, M, Sackmann, E
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Animals; Cell Membrane - metabolism; Cell Size; Cytology; Cytoskeleton - metabolism; Dictyostelium - cytology; Functions; Lipid Bilayers - metabolism; Liposomes - metabolism; Membrane Fluidity; Microscopy, Video; Models, Biological; Problem solving; Pseudopodia - physiology; Thermodynamics
• ISSN: 1063-651X; 1095-3787
• DOI: 10.1103/PhysRevE.62.3974

We present a theory showing that local shape instabilities of composite biological membranes, consisting of a lipid bilayer and an underlying actin cortex, can be triggered by a local softening of the membrane-associated cytoskeleton. A membrane containing such cortical defects can form blisters or invaginations, depending on external conditions. The theoretical predictions agree with observations provided by two sets of experiments: (i) microscopic observations of shape changes of giant vesicles with underlying shells of a thin actin network show the formation of local blisters and (ii) micropipet aspiration experiments of Dictyostelium discoideum cells in which we observed the formation of blisters in the aspirated cell part. In the latter experiments, the existence of a hole in the underlying cortex is confirmed by observation of the entrance of cell organelles into the blister. Our model may also be applied to the formation of lobopodia, fast-growing cell protrusions that play an important role in the locomotion and spreading of biological cells.