Identification of STKA-dependent genes in Dictyostelium discoideum

• Published in: Differentiation (London) 2000-10, Vol.66 (2), p.71-80
• Main Authors: Loughran, Gary, Pinter, Katalin, Newell, Peter C., Gross, Julian D.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cell Differentiation; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Cloning, Molecular; Dictyostelium - cytology; Dictyostelium - genetics; Dictyostelium - physiology; Dictyostelium discoideum; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Gene Library; Heterogeneous-Nuclear Ribonucleoproteins; hnRNA; Humans; Metalloendopeptidases - genetics; Molecular and cellular biology; Molecular Sequence Data; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Protozoan Proteins - genetics; Ribonucleoproteins - chemistry; Ribonucleoproteins - genetics; RNA, Messenger - genetics; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; spiA gene; Spores - physiology; stkA gene; STKA protein; Transcription, Genetic; zinc metalloproteinase
• ISSN: 0301-4681; 1432-0436
• DOI: 10.1046/j.1432-0436.2000.660202.x

During culmination of Dictyostelium aggregates, prespore and prestalk cells undergo terminal differentiation to form spores and a cellular stalk. Disruption of the cell-fate gene stkA leads to a phenotype in which all the cells destined to become spores end up as stalk cells. ‘Stalky’ mutants express normal levels of prespore cell transcripts but fail to produce the culmination-stage spore transcript spiA. The stkA gene encodes a putative GATA-type transcription factor (STKA). In order to identify possible downstream targets of STKA we used the technique of mRNA differential display and isolated four cDNA fragments that hybridise to mRNAs present during the later stages of development. All four gene tags were cloned and sequenced. mRNAs represented by these four sequence tags do not accumulate during culmination of ‘stalky’ cells and therefore must be specific to the spore pathway. By screening a cDNA library, longer cDNAs for all four were cloned and sequenced. Three of these contained complete protein-coding regions while only a partial cDNA was recovered for the fourth. One of the corresponding proteins has significant homology to a surface zinc metalloproteinase (GP63) of the protozoan parasite Leishmania, while another is closely related to a human pre-RNA binding protein (hnRNP R).