Orchestrated Transcription of Key Pathways in Arabidopsis by the Circadian Clock

Like most organisms, plants have endogenous biological clocks that coordinate internal events with the external environment. We used high-density oligonucleotide microarrays to examine gene expression in Arabidopsis and found that 6% of the more than 8000 genes on the array exhibited circadian chang...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2000-12, Vol.290 (5499), p.2110-2113
Main Authors: Harmer, Stacey L., Hogenesch, John B., Straume, Marty, Chang, Hur-Song, Han, Bin, Zhu, Tong, Wang, Xun, Kreps, Joel A., Kay, Steve A.
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - physiology
Arabidopsis: Research Articles
Biological and medical sciences
Biological Clocks - genetics
Carbohydrate Metabolism
Cell growth
Cell walls
Circadian Rhythm
Circadian rhythms
DNA
Enzymes
Epidermal cells
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Gene expression regulation
Gene Expression Regulation, Plant
Genes
Genes, Plant
Genetic aspects
Genetic regulation
Hypocotyls
Light
Molecular and cellular biology
Molecular genetics
Nitrogen - metabolism
Oligonucleotide Array Sequence Analysis
Photosynthesis - genetics
Photosynthetic Reaction Center Complex Proteins - genetics
Plant cells
Plants
Plants (Organisms)
Plants, Genetically Modified
Promoter Regions, Genetic
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Plant - genetics
RNA, Plant - metabolism
Sugars
Sulfur - metabolism
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Like most organisms, plants have endogenous biological clocks that coordinate internal events with the external environment. We used high-density oligonucleotide microarrays to examine gene expression in Arabidopsis and found that 6% of the more than 8000 genes on the array exhibited circadian changes in steady-state messenger RNA levels. Clusters of circadian-regulated genes were found in pathways involved in plant responses to light and other key metabolic pathways. Computational analysis of cycling genes allowed the identification of a highly conserved promoter motif that we found to be required for circadian control of gene expression. Our study presents a comprehensive view of the temporal compartmentalization of physiological pathways by the circadian clock in a eukaryote.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.290.5499.2110