Interleukin 1 receptor antagonist in human epidermis and cultured keratinocytes

Interleukin 1 (IL-1), present in high amounts in normal human skin without any sign of inflammation, suggests a complex mechanism by which its bioactivity is regulated. The specific receptor antagonist of IL-1 (IL-1ra) was analyzed in human skin, sweat and cultured keratinocytes. Extracts of both sk...

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Bibliographic Details
Published in:FEBS letters 1991-12, Vol.294 (1), p.137-140
Main Authors: Gruaz-Chatellard, D., Baumberger, C., Saurat, J.-H., Dayer, J.-M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Northern
Cell Line
Cell receptors
Cell structures and functions
Cells, Cultured
Epidermis - chemistry
Epidermis - physiology
Fundamental and applied biological sciences. Psychology
Human epidermis
Humans
Interleukin 1 Receptor Antagonist Protein
Interleukin-1
Interleukin-1 - analysis
Interleukin-1 - metabolism
Interleukin-1 receptor antagonist
Keratinocyte
Keratinocytes - chemistry
Keratinocytes - physiology
Kinetics
Miscellaneous
Molecular and cellular biology
Proteins - analysis
Proteins - genetics
Receptors, Immunologic - metabolism
Receptors, Interleukin-1
RNA - genetics
RNA - isolation & purification
Sialoglycoproteins
Sweat - chemistry
Sweat - physiology
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Summary:Interleukin 1 (IL-1), present in high amounts in normal human skin without any sign of inflammation, suggests a complex mechanism by which its bioactivity is regulated. The specific receptor antagonist of IL-1 (IL-1ra) was analyzed in human skin, sweat and cultured keratinocytes. Extracts of both skin and cultured keratinocytes blocked the binding of [ 125I]IL-1 to its receptor whereas sweat did not. The inhibitory activity was cell-associated, was not secreted by cultured keratinocytes, and IL-1ra mRNA was identified in these cells. There was an inverse relationship between the level of IL-1ra and that of IL-1α and β since extracts of differentiating keratinocytes (DK) and higher IL-1ra levels and expressed more mRNA for IL-1ra than non-differentiated keratinocytes (NDK), whereas NDK contained 4 times more IL-1α and β proteins than DK. This association of cell differentiation with a shift in agonist/antagonist ratio might be related to important autocrine or paracrine functions of IL-1 in normal and inflamed human skin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)81360-K