Delayed effects of pyridostigmine and exercise training on acetylcholinesterase and muscle tension in mouse lower extremity

In this study, the interactive effects of pyridostigmine, a pretreatment drug against nerve agents, and exercise training on muscle tension were investigated in the mouse lower extremity anterior muscular compartment by dorsiflexion of the foot with stimulation of the peroneal nerve. Acetylcholinest...

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Bibliographic Details
Published in:Archives of toxicology 2000-11, Vol.74 (9), p.539-546
Main Authors: VERMA-AHUJA, S, HUSAIN, K, VERHULST, S, ESPINOSA, J. A, SOMANI, S. M
Format: Article
Language:English
Subjects:
Acetylcholinesterase - metabolism
Animals
Biological and medical sciences
Cholinesterase Inhibitors - pharmacology
Creatine Kinase - metabolism
Drug toxicity and drugs side effects treatment
Electric Stimulation
Leg - physiology
Lipid Peroxidation - drug effects
Male
Malondialdehyde - metabolism
Medical sciences
Mice
Muscle Contraction - drug effects
Muscle Proteins - metabolism
Muscle, Skeletal - drug effects
Muscle, Skeletal - enzymology
Oxygen Consumption
Pharmacology. Drug treatments
Physical Conditioning, Animal - physiology
pyridostigmine
Pyridostigmine Bromide - pharmacology
Toxicity: nervous system and muscle
Weight Gain - drug effects
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Summary:In this study, the interactive effects of pyridostigmine, a pretreatment drug against nerve agents, and exercise training on muscle tension were investigated in the mouse lower extremity anterior muscular compartment by dorsiflexion of the foot with stimulation of the peroneal nerve. Acetylcholinesterase (AChE), lipid peroxidation (in terms of the end-product malondialdehyde, MDA) and creatine phosphokinase (CPK) activity in the muscle were correlated with muscle tension. Male NIH Swiss mice were divided into four groups and treated as follows: (1) sedentary control; (2) pyridostigmine (1.2 mg/kg orally) daily for the 5th and 6th weeks; (3) exercise training for 10 weeks; and (4) pyridostigmine plus exercise training for 10 weeks. Experiments on muscle tension were conducted 4 weeks after the last dose of pyridostigmine or saline and 24 h after exercise. The muscle tension was measured in right and left legs using a tension transduction device connected to a polygraph. After muscle tension recording, mice were killed, blood and triceps muscle were isolated, and plasma CPK and muscle AChE activities, and MDA were determined. There was a significant increase in the muscle tension (P
ISSN:0340-5761
1432-0738
DOI:10.1007/s002040000169