Kinetics of allantoin metabolism in sheep

The kinetics of allantoin metabolism were studied in rumen-cannulated sheep by means of a single intravenous injection of [4,5-14C]allantoin. The decline in the specific radioactivity of allantoin in plasma following the injection of tracer was best described by a double exponential function, indica...

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Bibliographic Details
Published in:British journal of nutrition 2000-11, Vol.84 (5), p.629-634
Main Authors: Kahn, L. P., Nolan, J. V.
Format: Article
Language:English
Subjects:
Allantoin
Allantoin - blood
Allantoin - metabolism
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid
Fundamental and applied biological sciences. Psychology
HPLC
Injections, Intravenous
Male
Microbial protein
Purine
Radioactive Tracers
Sheep
Sheep - metabolism
Stomach
Vertebrates: digestive system
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Summary:The kinetics of allantoin metabolism were studied in rumen-cannulated sheep by means of a single intravenous injection of [4,5-14C]allantoin. The decline in the specific radioactivity of allantoin in plasma following the injection of tracer was best described by a double exponential function, indicating that allantoin moves in and out of two or more kinetically distinct compartments. Sequestering of tracer in secondary or tertiary compartments in the body water appears likely to have resulted in overestimation of net flux of allantoin through the blood in the present study. In future studies, sampling of blood for several days after administration of tracer should alleviate this problem. About 80 % of the [14C]allantoin injected was recovered as [14C]allantoin in urine during the 12 h after tracer injection, increasing to 94 % after 4 d. Allantoin-C also passed through the blood bicarbonate pool, suggesting that allantoin is degraded in the gastrointestinal tract. A small amount of allantoin-C (4 % of the net flux of allantoin through the blood pool) was apparently degraded to form bicarbonate-C in the rumen and postruminally, and subsequently appeared in blood bicarbonate-C. Transfer of allantoin-C into the rumen via saliva was insignificant. In view of these findings, the net flux of allantoin through the blood should be a better predictor of rumen microbial outflow than urinary allantoin excretion, because urinary excretion of purine derivatives must be adjusted for conversion of allantoin-C to blood bicarbonate when used to predict the flow of microbial protein from the rumen.
ISSN:0007-1145
1475-2662
DOI:10.1017/S0007114500001963