Biostimulation of bone marrow cells with a diode soft laser

In recent years, the use of low‐intensity red light in regeneration of soft tissue has been increasingly pursued. As far as hard tissue is concerned, the biostimulating effect of laser has already been demonstrated successfully in more rapid healing of tibial bone fractures in mice at a dosage of 2....

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Bibliographic Details
Published in:Clinical oral implants research 2000-12, Vol.11 (6), p.540-545
Main Authors: Dörtbudak, Orhun, Haas, Robert, Mailath-Pokorny, Georg
Format: Article
Language:English
Subjects:
Animals
biostimulation
Bone Development - radiation effects
bone marrow cells
Bone Marrow Cells - cytology
Bone Marrow Cells - radiation effects
Bone Regeneration - radiation effects
Cells, Cultured
Dentistry
diode soft laser
Fluorescent Dyes
Lasers
Male
Mesoderm - cytology
Osteoblasts - cytology
Osteoblasts - radiation effects
Rats
Rats, Wistar
Statistics as Topic
Tetracycline
Time Factors
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Summary:In recent years, the use of low‐intensity red light in regeneration of soft tissue has been increasingly pursued. As far as hard tissue is concerned, the biostimulating effect of laser has already been demonstrated successfully in more rapid healing of tibial bone fractures in mice at a dosage of 2.4 J. However, the effect of light of a low dose laser directly on osteoblasts has not been investigated yet. The aim of this study was to determine the effect of continuous wave diode laser irradiation on osteoblasts derived mesenchymal cells. Three groups of 10 cultures each were irradiated 3 times (days 3, 5, 7) with a pulsed diode soft laser with a wavelength of 690 nm for 60 s. Another 3 groups of 10 cultures each were used as control groups. A newly developed method employing the fluorescent antibiotic tetracycline was used to compare bone growth on these culture substrates after a period of 8, 12 and 16 days, respectively. It was found that all lased cultures demonstrated significantly more fluorescent bone deposits than the non‐lased cultures. The difference was significant, as tested by the Tukey Test (P
ISSN:0905-7161
1600-0501
DOI:10.1034/j.1600-0501.2000.011006540.x